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PCR-based identification of hyperthermophilic archaea of the family Thermococcaceae.


ABSTRACT: A method for rapid detection and identification of hyperthermophilic archaea of the family Thermococcaceae based on PCR amplification of 16S rRNA gene fragments with primers TcPc 173F (5'-TCCCCCATAGGYCTGRGGTACTGGAAGGTC-3') and TcPc 589R (5'-GCCGTGRGATTTCGCCAGGGACTTACGGGC-3') was developed and used for identification of new isolates.

SUBMITTER: Slobodkina GB 

PROVIDER: S-EPMC520901 | biostudies-literature | 2004 Sep

REPOSITORIES: biostudies-literature

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PCR-based identification of hyperthermophilic archaea of the family Thermococcaceae.

Slobodkina Galina B GB   Chernyh Nikolai A NA   Slobodkin Alexander I AI   Subbotina Irina V IV   Bonch-Osmolovskaya Elizaveta A EA   Lebedinsky Alexander V AV  

Applied and environmental microbiology 20040901 9


A method for rapid detection and identification of hyperthermophilic archaea of the family Thermococcaceae based on PCR amplification of 16S rRNA gene fragments with primers TcPc 173F (5'-TCCCCCATAGGYCTGRGGTACTGGAAGGTC-3') and TcPc 589R (5'-GCCGTGRGATTTCGCCAGGGACTTACGGGC-3') was developed and used for identification of new isolates. ...[more]

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