Single-nucleotide polymorphism discovery by targeted DNA photocleavage.
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ABSTRACT:
SUBMITTER: Hart JR
PROVIDER: S-EPMC521117 | biostudies-literature | 2004 Sep
REPOSITORIES: biostudies-literature
Publications
Single-nucleotide polymorphism discovery by targeted DNA photocleavage.
Hart Jonathan R JR Johnson Martin D MD Barton Jacqueline K JK
Proceedings of the National Academy of Sciences of the United States of America 20040921 39
Single-nucleotide polymorphisms are the largest source of genetic variation in humans. We report a method for the discovery of single-nucleotide polymorphisms within genomic DNA. Pooled genomic samples are amplified, denatured, and annealed to generate mismatches at polymorphic DNA sites. Upon photoactivation, these DNA mismatches are then cleaved site-specifically by using a small molecular probe, a bulky metallointercalator, Rhchrysi or Rhphzi. Fluorescent labeling of the cleaved products and ...[more]
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