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The assessment of protective behavioral strategies: comparing the absolute frequency and contingent frequency response scales.

ABSTRACT: The purpose of the present studies was to examine the effects of response scale on the observed relationships between protective behavioral strategies (PBS) measures and alcohol-related outcomes. We reasoned that an "absolute frequency" scale (stem: "how many times …"; response scale: 0 times to 11+ times) conflates the frequency of using PBS with the frequency of consuming alcohol; thus, we hypothesized that the use of an absolute frequency response scale would result in positive relationships between types of PBS and alcohol-related outcomes. Alternatively, a "contingent frequency" scale (stem: "When drinking … how often …"; response scale: never to always) does not conflate frequency of alcohol use with use of PBS; therefore, we hypothesized that use of a contingent frequency scale would result in negative relationships between use of PBS and alcohol-related outcomes. Two published measures of PBS were used across studies: the Protective Behavioral Strategies Survey (PBSS) and the Strategy Questionnaire (SQ). Across three studies, we demonstrate that when measured using a contingent frequency response scale, PBS measures relate negatively to alcohol-related outcomes in a theoretically consistent manner; however, when PBS measures were measured on an absolute frequency response scale, they were nonsignificantly or positively related to alcohol-related outcomes. We discuss the implications of these findings for the assessment of PBS.

SUBMITTER: Kite BA

PROVIDER: S-EPMC5216259 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature

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The assessment of protective behavioral strategies: comparing the absolute frequency and contingent frequency response scales.

Kite Benjamin A BA Pearson Matthew R MR Henson James M JM

Psychology of addictive behaviors : journal of the Society of Psychologists in Addictive Behaviors 20130225 4

The purpose of the present studies was to examine the effects of response scale on the observed relationships between protective behavioral strategies (PBS) measures and alcohol-related outcomes. We reasoned that an "absolute frequency" scale (stem: "how many times …"; response scale: 0 times to 11+ times) conflates the frequency of using PBS with the frequency of consuming alcohol; thus, we hypothesized that the use of an absolute frequency response scale would result in positive relationships ...[more]

PMID: 23438243

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Project description:There is a great interest in reliable ways to obtain absolute protein abundances at a proteome-wide scale. To this end, label-free LC-MS/MS quantification methods have been proposed where all identified proteins are assigned an estimated abundance. Several variants of this quantification approach have been presented, based on either the number of spectral counts per protein or MS1 peak intensities. Equipped with several datasets representing real biological environments, containing a high number of accurately quantified reference proteins, we evaluate five popular low cost and easily implemented quantification methods (APEX, emPAI, iBAQ, Top3 and MeanInt). Our results demonstrate considerably improved abundance estimates upon implementing accurately quantified reference proteins; i.e. using spiked in SIS peptides or a standard protein mix, to generate a properly calibrated quantification model. We show that only the Top3 method is directly proportional to protein abundance over the full quantification range and is the preferred method in the absence of reference protein measurements. Additionally, we demonstrate that spectral count based quantification methods are associated with higher errors than MS1 peak intensity based methods. Furthermore, we investigate the impact of mis-cleaved, modified and shared peptides as well as protein size and the number of employed reference proteins on quantification accuracy. The .raw data submitted to PRIDE correspond to replicate DDA LC-MS/MS analysis of the UPS2 mix (Universal Proteomics Standard, UPS2, Sigma-Aldrich), as well as triplicate DDA LC-MS/MS analysis of the UPS2 mix spiked into samples from the following organisms M. pneumoniae, L. interrogans and D. melanogaster. The accompanying R-script called calculatePQIs.R was used to calculate the different Quantification Indices, provided that the peptide XICs or Spectral Counts have been loaded into a data.frame R object (which can be produced by SafeQuant).

Dataset Information

The assessment of protective behavioral strategies: comparing the absolute frequency and contingent frequency response scales.

Publications

The assessment of protective behavioral strategies: comparing the absolute frequency and contingent frequency response scales.

Similar Datasets

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