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Disulfide isomerase-like protein AtPDIL1-2 is a good candidate for trichlorophenol phytodetoxification.


ABSTRACT: Trichlorophenol (TCP) is a widely used and persistent environmentally toxic compound that poses a carcinogenic risk to humans. Phytoremediation is a proficient cleanup technology for organic pollutants. In this study, we found that the disulfide isomerase-like protein AtPDIL1-2 in plants is a good candidate for enhancing 2,4,6-TCP phytoremediation. The expression of AtPDIL1-2 in Arabidopsis was induced by 2,4,6-TCP. The heterologously expressed AtPDIL1-2 in Escherichia coli exhibited both oxidase and isomerase activities as protein disulfide isomerase and improved bacteria tolerance to 2,4,6-TCP. Further research revealed that transgenic tobacco overexpressing AtPDIL1-2 was more tolerant to high concentrations of 2,4,6-TCP and removed the toxic compound at far greater rates than the control plants. To elucidate the mechanism of action of AtPDIL1-2, we investigated the chemical interaction of AtPDIL1-2 with 2,4,6-TCP for the first time. HPLC analysis implied that AtPDIL1-2 exerts a TCP-binding activity. A suitable configuration of AtPDIL1-2-TCP binding was obtained by molecular docking studies using the AutoDock program. It predicted that the TCP binding site is located in the b-b' domain of AtPDIL1-2 and that His254 of the protein is critical for the binding interaction. These findings imply that AtPDIL1-2 can be used for TCP detoxification by the way of overexpression in plants.

SUBMITTER: Peng RH 

PROVIDER: S-EPMC5216352 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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Disulfide isomerase-like protein AtPDIL1-2 is a good candidate for trichlorophenol phytodetoxification.

Peng Ri-He RH   Qiu Jin J   Tian Yong-Sheng YS   Gao Jian-Jie JJ   Han Hong-Juan HJ   Fu Xiao-Yan XY   Zhu Bo B   Xu Jing J   Wang Bo B   Li Zhen-Jun ZJ   Wang Li-Juan LJ   Yao Quan-Hong QH  

Scientific reports 20170106


Trichlorophenol (TCP) is a widely used and persistent environmentally toxic compound that poses a carcinogenic risk to humans. Phytoremediation is a proficient cleanup technology for organic pollutants. In this study, we found that the disulfide isomerase-like protein AtPDIL1-2 in plants is a good candidate for enhancing 2,4,6-TCP phytoremediation. The expression of AtPDIL1-2 in Arabidopsis was induced by 2,4,6-TCP. The heterologously expressed AtPDIL1-2 in Escherichia coli exhibited both oxidas  ...[more]

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