Gene profile of fibroblasts identify relation of CCL8 with idiopathic pulmonary fibrosis.

ABSTRACT:

Background

Idiopathic pulmonary fibrosis (IPF) is characterized by the complex interaction of cells involved in chronic inflammation and fibrosis. Global gene expression of a homogenous cell population will identify novel candidate genes.

Methods

Gene expression of fibroblasts derived from lung tissues (8 IPF and 4 controls) was profiled, and ontology and functional pathway were analyzed in the genes exhibiting >2 absolute fold changes with p-values?ResultsOne hundred seventy eight genes differentially expressed and 15 genes exhibited >10-fold change. Among them, 13 were novel in relation with IPF. CCL8 expression was 22.8-fold higher in IPF fibroblasts. The levels of CCL8 mRNA and protein were 3 and 9-fold higher in 14 IPF fibroblasts than those in 10 control fibroblasts by real-time PCR and ELISA (p?=?0.022 and p?=?0.026, respectively). The CCL8 concentrations in BAL fluid was significantly higher in 86 patients with IPF than those in 41 controls, and other interstitial lung diseases including non-specific interstitial pneumonia (n?=?22), hypersensitivity pneumonitis (n?=?20) and sarcoidosis (n?=?19) (p?28.61 pg/mL showed shorter survival compared to those with lower levels (p?=?0.012). CCL8 was expressed by ?-SMA-positive cells in the interstitium of IPF.

Conclusions

Transcriptome analysis identified several novel IPF-related genes. Among them, CCL8 is a candidate molecule for the differential diagnosis and prediction of survival.

SUBMITTER: Lee JU 

PROVIDER: S-EPMC5216573 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature