Ontology highlight
ABSTRACT:
SUBMITTER: Miner BE
PROVIDER: S-EPMC521679 | biostudies-literature | 2004 Sep
REPOSITORIES: biostudies-literature
Miner Brooks E BE Stöger Reinhard J RJ Burden Alice F AF Laird Charles D CD Hansen R Scott RS
Nucleic acids research 20040930 17
PCR amplification of limited amounts of DNA template carries an increased risk of product redundancy and contamination. We use molecular barcoding to label each genomic DNA template with an individual sequence tag prior to PCR amplification. In addition, we include molecular 'batch-stamps' that effectively label each genomic template with a sample ID and analysis date. This highly sensitive method identifies redundant and contaminant sequences and serves as a reliable method for positive identif ...[more]