Ontology highlight
ABSTRACT:
SUBMITTER: Timachi MH
PROVIDER: S-EPMC5216877 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
eLife 20170104
ABC exporters pump substrates across the membrane by coupling ATP-driven movements of nucleotide binding domains (NBDs) to the transmembrane domains (TMDs), which switch between inward- and outward-facing (IF, OF) orientations. DEER measurements on the heterodimeric ABC exporter TM287/288 from <i>Thermotoga maritima</i>, which contains a non-canonical ATP binding site, revealed that in the presence of nucleotides the transporter exists in an IF/OF equilibrium. While ATP binding was sufficient to ...[more]