Comparing Simplification Strategies for the Skeletal Muscle Proteome.
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ABSTRACT:
SUBMITTER: Geary B
PROVIDER: S-EPMC5217366 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
Publications
Comparing Simplification Strategies for the Skeletal Muscle Proteome.
Geary Bethany B Young Iain S IS Cash Phillip P Whitfield Phillip D PD Doherty Mary K MK
Proteomes 20160302 1
Skeletal muscle is a complex tissue that is dominated by the presence of a few abundant proteins. This wide dynamic range can mask the presence of lower abundance proteins, which can be a confounding factor in large-scale proteomic experiments. In this study, we have investigated a number of pre-fractionation methods, at both the protein and peptide level, for the characterization of the skeletal muscle proteome. The analyses revealed that the use of OFFGEL isoelectric focusing yielded the large ...[more]
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