Perspective: revisiting the field dependence of TROSY sensitivity.

ABSTRACT: The discovery of the TROSY effect (Pervushin et al. in Proc Natl Acad Sci USA 94:12366-12371, 1997) for reducing transverse relaxation and line sharpening through selecting pathways in which dipole-dipole and CSA Hamiltonians partially cancel each other had a tremendous impact on solution NMR studies of macromolecules. Together with the methyl TROSY (Tugarinov and Kay in J Biomol NMR 28:165-172, 2004) it enabled structural and functional studies of significantly larger systems. The optimal field strengths for TROSY have been estimated to be on spectrometers operating around 900 MHz (21.14 T) for the ¹H_N TROSY (Pervushin et al. in Proc Natl Acad Sci USA 94:12366-12371, 1997) while the aromatic ¹³C (¹³C_aro) TROSY is posited to be optimal

SUBMITTER: Takeuchi K 

PROVIDER: S-EPMC5218892 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature