Unknown

Dataset Information

0

A universal telomerase RNA core structure includes structured motifs required for binding the telomerase reverse transcriptase protein.


ABSTRACT: Telomerase synthesizes telomeric DNA by copying a short template sequence within its telomerase RNA component. We delineated nucleotides and base-pairings within a previously mapped central domain of the Saccharomyces cerevisiae telomerase RNA (TLC1) that are important for telomerase function and for binding to the telomerase catalytic protein Est2p. Phylogenetic comparison of telomerase RNA sequences from several budding yeasts revealed a core structure common to Saccharomyces and Kluyveromyces yeast species. We show that in this structure three conserved sequences interact to provide a binding site for Est2p positioned near the template. These results, combined with previous studies on telomerase RNAs from other budding yeasts, vertebrates, and ciliates, define a minimal universal core for telomerase RNAs.

SUBMITTER: Lin J 

PROVIDER: S-EPMC522012 | biostudies-literature | 2004 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

A universal telomerase RNA core structure includes structured motifs required for binding the telomerase reverse transcriptase protein.

Lin Jue J   Ly Hinh H   Hussain Arif A   Abraham Mira M   Pearl Sivan S   Tzfati Yehuda Y   Parslow Tristram G TG   Blackburn Elizabeth H EH  

Proceedings of the National Academy of Sciences of the United States of America 20040915 41


Telomerase synthesizes telomeric DNA by copying a short template sequence within its telomerase RNA component. We delineated nucleotides and base-pairings within a previously mapped central domain of the Saccharomyces cerevisiae telomerase RNA (TLC1) that are important for telomerase function and for binding to the telomerase catalytic protein Est2p. Phylogenetic comparison of telomerase RNA sequences from several budding yeasts revealed a core structure common to Saccharomyces and Kluyveromyces  ...[more]

Similar Datasets

| S-EPMC2913471 | biostudies-other
| S-EPMC507002 | biostudies-literature
| S-EPMC4714691 | biostudies-literature
| S-EPMC2561060 | biostudies-literature
| S-EPMC2950546 | biostudies-literature
2024-07-11 | GSE145659 | GEO
| S-EPMC262686 | biostudies-literature
| S-EPMC2929889 | biostudies-other
| S-EPMC7898544 | biostudies-literature
| S-EPMC7526981 | biostudies-literature