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Cloning and expression of clt genes encoding milk-clotting proteases from Myxococcus xanthus 422.

ABSTRACT: The screening of a gene library of the milk-clotting strain Myxococcus xanthus 422 constructed in Escherichia coli allowed the description of eight positive clones containing 26 open reading frames. Only three of them (cltA, cltB, and cltC) encoded proteins that exhibited intracellular milk-clotting ability in E. coli, Saccharomyces cerevisiae, and Pichia pastoris expression systems.

SUBMITTER: Poza M

PROVIDER: S-EPMC522063 | biostudies-literature | 2004 Oct

REPOSITORIES: biostudies-literature

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Cloning and expression of clt genes encoding milk-clotting proteases from Myxococcus xanthus 422.

Poza M M Prieto-Alcedo M M Sieiro C C Villa T G TG

Applied and environmental microbiology 20041001 10

The screening of a gene library of the milk-clotting strain Myxococcus xanthus 422 constructed in Escherichia coli allowed the description of eight positive clones containing 26 open reading frames. Only three of them (cltA, cltB, and cltC) encoded proteins that exhibited intracellular milk-clotting ability in E. coli, Saccharomyces cerevisiae, and Pichia pastoris expression systems. ...[more]

PMID: 15466588

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