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Bacterial selection for biological control of plant disease: criterion determination and validation.

ABSTRACT: This study aimed to evaluate the biocontrol potential of bacteria isolated from different plant species and soils. The production of compounds related to phytopathogen biocontrol and/or promotion of plant growth in bacterial isolates was evaluated by measuring the production of antimicrobial compounds (ammonia and antibiosis) and hydrolytic enzymes (amylases, lipases, proteases, and chitinases) and phosphate solubilization. Of the 1219 bacterial isolates, 92% produced one or more of the eight compounds evaluated, but only 1% of the isolates produced all the compounds. Proteolytic activity was most frequently observed among the bacterial isolates. Among the compounds which often determine the success of biocontrol, 43% produced compounds which inhibit mycelial growth of Monilinia fructicola, but only 11% hydrolyzed chitin. Bacteria from different plant species (rhizosphere or phylloplane) exhibited differences in the ability to produce the compounds evaluated. Most bacterial isolates with biocontrol potential were isolated from rhizospheric soil. The most efficient bacteria (producing at least five compounds related to phytopathogen biocontrol and/or plant growth), 86 in total, were evaluated for their biocontrol potential by observing their ability to kill juvenile Mesocriconema xenoplax. Thus, we clearly observed that bacteria that produced more compounds related to phytopathogen biocontrol and/or plant growth had a higher efficacy for nematode biocontrol, which validated the selection strategy used.

SUBMITTER: Mota MS

PROVIDER: S-EPMC5220633 | biostudies-literature | 2017 Jan - Mar

REPOSITORIES: biostudies-literature

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Bacterial selection for biological control of plant disease: criterion determination and validation.

Mota Monalize Salete MS Gomes Cesar Bauer CB Souza Júnior Ismail Teodoro IT Moura Andréa Bittencourt AB

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] 20161004 1

This study aimed to evaluate the biocontrol potential of bacteria isolated from different plant species and soils. The production of compounds related to phytopathogen biocontrol and/or promotion of plant growth in bacterial isolates was evaluated by measuring the production of antimicrobial compounds (ammonia and antibiosis) and hydrolytic enzymes (amylases, lipases, proteases, and chitinases) and phosphate solubilization. Of the 1219 bacterial isolates, 92% produced one or more of the eight co ...[more]

PMID: 27765523

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Project description:Southern blight of tomato caused by Sclerotium rolfsii can cause severe plant mortality and yield losses. The use of rhizobacteria for the biological control of Southern blight disease is a potent alternative to chemical fungicides. Although rhizobacteria are prolific candidates, comprehensive reports regarding their use in tomato disease management are limited. The present study screened six rhizobacterial strains for antagonism against S. rolfsii in dual culture and culture filtrate assays. The selected promising strains were tested further for plant-growth-promoting and biocontrol potentials under in vitro, greenhouse, and field conditions. Of the six strains screened, Stenotrophomonas maltophilia PPB3 and Bacillus subtilis PPB9 showed the superior performance displaying the highest antagonism against S. rolfsii in dual culture (PPB3 88% and PPB9 71% inhibition), and culture filtrate assays (PPB3 53-100% and PPB9 54-100% inhibition at various concentrations). Oxalic acid produced by S. rolfsii was significantly inhibited by both rhizobacteria and supported their growth as a carbon source. The strains produced hydrogen cyanide, chitinases, siderophores, biofilm, and indole acetic acid. They showed the potential to solubilize phosphate and fix nitrogen. Seed treatment with S. maltophilia PPB3 and B. subtilis PPB9 improved seed germination and tomato seedling vigour. Significant increases in plant growth, chlorophyll contents, and N, P, and K concentrations were attained in bacterized plants compared to non-treated controls. The application of antagonists on container-grown seedlings in a greenhouse environment and field-grown tomato plants reduced symptoms of damping-off and Southern blight. The sclerotial counts decreased significantly in these soils. Bacteria-inoculated plants had a higher yield than those in the non-treated control. Bacteria colonized the entire roots, and their populations increased significantly in the protected plants. The results show the potential capabilities of S. maltophilia PPB3 and B. subtilis PPB9 for growth promotion, nutrient acquisition, and biocontrol of southern blight disease in tomatoes.

Dataset Information

Bacterial selection for biological control of plant disease: criterion determination and validation.

Publications

Bacterial selection for biological control of plant disease: criterion determination and validation.

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