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Genetic identification of a putative vinyl chloride reductase in Dehalococcoides sp. strain BAV1.

ABSTRACT: Dehalococcoides sp. strain BAV1 couples growth with the reductive dechlorination of vinyl chloride (VC) to ethene. Degenerate primers targeting conserved regions in reductive dehalogenase (RDase) genes were designed and used to PCR amplify putative RDase genes from strain BAV1. Seven unique RDase gene fragments were identified. Transcription analysis of VC-grown BAV1 cultures suggested that bvcA was involved in VC reductive dechlorination, and the complete sequence of bvcA was obtained. bvcA was absent in Dehalococcoides isolates that failed to respire VC, yet was detected in four of eight VC-respiring mixed cultures.

SUBMITTER: Krajmalnik-Brown R

PROVIDER: S-EPMC522117 | biostudies-literature | 2004 Oct

REPOSITORIES: biostudies-literature

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Genetic identification of a putative vinyl chloride reductase in Dehalococcoides sp. strain BAV1.

Krajmalnik-Brown Rosa R Hölscher Tina T Thomson Ivy N IN Saunders F Michael FM Ritalahti Kirsti M KM Löffler Frank E FE

Applied and environmental microbiology 20041001 10

Dehalococcoides sp. strain BAV1 couples growth with the reductive dechlorination of vinyl chloride (VC) to ethene. Degenerate primers targeting conserved regions in reductive dehalogenase (RDase) genes were designed and used to PCR amplify putative RDase genes from strain BAV1. Seven unique RDase gene fragments were identified. Transcription analysis of VC-grown BAV1 cultures suggested that bvcA was involved in VC reductive dechlorination, and the complete sequence of bvcA was obtained. bvcA was ...[more]

PMID: 15466590

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