Efficient biosynthesis of a Cecropin A-melittin mutant in Bacillus subtilis WB700.
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ABSTRACT:
SUBMITTER: Ji S
PROVIDER: S-EPMC5223193 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
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Efficient biosynthesis of a Cecropin A-melittin mutant in Bacillus subtilis WB700.
Ji Shengyue S Li Weili W Baloch Abdul Rasheed AR Wang Meng M Li Hengxin H Cao Binyun B Zhang Hongfu H
Scientific reports 20170110
The efficient production of antimicrobial peptides (AMPs) for clinical applications has attracted the attention of the scientific community. To develop a novel microbial cell factory for the efficient biosynthesis of a cecropin A-melittin mutant (CAM-W), a recombinant Bacillus subtilis WB700 expression system was genetically modified with a novel vector, including a fusion gene encoding CAM-W, the autoprotease EDDIE and the signal peptide SacB under the control of the maltose-inducible promoter ...[more]
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