Dataset Information

Cloning and characterization of an ABA-independent DREB transcription factor gene, HcDREB2, in Hemarthria compressa.

ABSTRACT: Hemarthria compressa is a stoloniferous perennial tropical forage grass with a wide geographic distribution; however, environmental stress has a great influence on its growth. The DREB transcription factor family genes contains candidate genes for improving plant stress tolerance.From cold-treated H. compressa plants, a putative DREB2 gene (HcDREB2) was cloned using the RACE-PCR method. HcDREB2 was 1296 bp in length and encoded a putative protein 264 amino acid residues long. HcDREB2 shared the highest sequence identity with DREB2 in sorghum. The expression of HcDREB2 was independent of ABA treatment, but inducible by low temperatures as well as drought and high salinity treatments. Yeast one-hybrid assays showed that HcDREB2 directly bound the DRE cis-acting element to transactivate the expression of the downstream reporter genes.HcDREB2, a stress-inducible but ABA-independent transcription factor gene, can transactivate downstream genes by binding to the DRE cis-element. The current results are a foundation for making use of this stress tolerance gene in future H. compressa studies.

SUBMITTER: Chen Y

PROVIDER: S-EPMC5224587 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Publications

Cloning and characterization of an ABA-independent DREB transcription factor gene, <i>HcDREB2</i>, in <i>Hemarthria compressa</i>.

Chen Yongxia Y Huang Linkai L Yan Haidong H Zhang Xinquan X Xu Bin B Ma Xiao X

Hereditas 20160408

<h4>Background</h4><i>Hemarthria compressa</i> is a stoloniferous perennial tropical forage grass with a wide geographic distribution; however, environmental stress has a great influence on its growth. The DREB transcription factor family genes contains candidate genes for improving plant stress tolerance.<h4>Results</h4>From cold-treated <i>H. compressa</i> plants, a putative <i>DREB2</i> gene (<i>HcDREB2</i>) was cloned using the RACE-PCR method. <i>HcDREB2</i> was 1296 bp in length and encode ...[more]

PMID: 28096765

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Project description:BACKGROUND: TCP proteins are plant-specific transcription factors, which are known to have a wide range of functions in different plant species such as in leaf development, flower symmetry, shoot branching, and senescence. Only a small number of TCP genes has been characterised from tomato (Solanum lycopersicum). Here we report several functional features of the members of the entire family present in the tomato genome. RESULTS: We have identified 30 Solanum lycopersicum SlTCP genes, most of which have not been described before. Phylogenetic analysis clearly distinguishes two homology classes of the SlTCP transcription factor family - class I and class II. Class II differentiates in two subclasses, the CIN-TCP subclass and the CYC/TB1 subclass, involved in leaf development and axillary shoots formation, respectively. The expression patterns of all members were determined by quantitative PCR. Several SlTCP genes, like SlTCP12, SlTCP15 and SlTCP18 are preferentially expressed in the tomato fruit, suggesting a role during fruit development or ripening. These genes are regulated by RIN (RIPENING INHIBITOR), CNR (COLORLESS NON-RIPENING) and SlAP2a (APETALA2a) proteins, which are transcription factors with key roles in ripening. With a yeast one-hybrid assay we demonstrated that RIN binds the promoter fragments of SlTCP12, SlTCP15 and SlTCP18, and that CNR binds the SlTCP18 promoter. This data strongly suggests that these class I SlTCP proteins are involved in ripening. Furthermore, we demonstrate that SlTCPs bind the promoter fragments of members of their own family, indicating that they regulate each other. Additional yeast one-hybrid studies performed with Arabidopsis transcription factors revealed binding of the promoter fragments by proteins involved in the ethylene signal transduction pathway, contributing to the idea that these SlTCP genes are involved in the ripening process. Yeast two-hybrid data shows that SlTCP proteins can form homo and heterodimers, suggesting that they act together in order to form functional protein complexes and together regulate developmental processes in tomato. CONCLUSIONS: The comprehensive analysis we performed, like phylogenetic analysis, expression studies, identification of the upstream regulators and the dimerization specificity of the tomato TCP transcription factor family provides the basis for functional studies to reveal the role of this family in tomato development.

Dataset Information

Cloning and characterization of an ABA-independent DREB transcription factor gene, HcDREB2, in Hemarthria compressa.

Publications

Cloning and characterization of an ABA-independent DREB transcription factor gene, <i>HcDREB2</i>, in <i>Hemarthria compressa</i>.

Similar Datasets

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