Ontology highlight
ABSTRACT:
SUBMITTER: Zakas PM
PROVIDER: S-EPMC5225049 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
Zakas Philip M PM Brown Harrison C HC Knight Kristopher K Meeks Shannon L SL Spencer H Trent HT Gaucher Eric A EA Doering Christopher B CB
Nature biotechnology 20160926 1
Optimization of a protein's pharmaceutical properties is usually carried out by rational design and/or directed evolution. Here we test an alternative approach based on ancestral sequence reconstruction. Using available genomic sequence data on coagulation factor VIII and predictive models of molecular evolution, we engineer protein variants with improved activity, stability, and biosynthesis potential and reduced inhibition by anti-drug antibodies. In principle, this approach can be applied to ...[more]