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Intravital multiphoton imaging of mouse tibialis anterior muscle.

ABSTRACT: Intravital imaging by multiphoton microscopy is a powerful tool to gain invaluable insight into tissue biology and function. Here, we provide a step-by-step tissue preparation protocol for imaging the mouse tibialis anterior skeletal muscle. Additionally, we include steps for jugular vein catheterization that allow for well-controlled intravenous reagent delivery. Preparation of the tibialis anterior muscle is minimally invasive, reducing the chances of inducing damage and inflammation prior to imaging. The tibialis anterior muscle is useful for imaging leukocyte interaction with vascular endothelium, and to understand muscle contraction biology. Importantly, this model can be easily adapted to study neuromuscular diseases and myopathies.

SUBMITTER: Lau J

PROVIDER: S-EPMC5226003 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Intravital multiphoton imaging of mouse tibialis anterior muscle.

Lau Jasmine J Goh Chi Ching CC Devi Sapna S Keeble Jo J See Peter P Ginhoux Florent F Ng Lai Guan LG

Intravital 20160408 2

Intravital imaging by multiphoton microscopy is a powerful tool to gain invaluable insight into tissue biology and function. Here, we provide a step-by-step tissue preparation protocol for imaging the mouse tibialis anterior skeletal muscle. Additionally, we include steps for jugular vein catheterization that allow for well-controlled intravenous reagent delivery. Preparation of the tibialis anterior muscle is minimally invasive, reducing the chances of inducing damage and inflammation prior to ...[more]

PMID: 28243520

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