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Elevated serum microRNA 483-5p levels may predict patients at risk of post-operative atrial fibrillation.


ABSTRACT:

Objectives

Post-operative atrial fibrillation (POAF) is the commonest post-operative cardiac arrhythmia, affecting ?1 in 3 patients undergoing coronary artery bypass grafting (CABG). Although its aetiology is complex, atrial substrate changes may pre-dispose to its onset. This study aims to ascertain the atrial microRNA signature of POAF and determine the potential for circulating microRNA as a pre-operative biomarker for this arrhythmia.

Methods

Thirty-four patients undergoing non-emergent, on-pump CABG were prospectively recruited. Right atrial biopsies were taken intra-operatively and snap frozen for RNA extraction. Plasma was obtained at 24 h pre-operatively and at 2 and 4 days post-operatively. POAF was defined by continuous Holter recording. Inter-group comparisons were performed using Student's t-test or analysis of variance as required. Receiver operating characteristic (ROC) analysis was used to determine the diagnostic accuracy of pre-operative serum miRNA as a POAF biomarker.

Results

Sixteen microRNAs were differentially expressed in the atrial myocardium of POAF patients when compared with those maintaining sinus rhythm. miR-208a was the most underexpressed [fold change (FC) = 2.458] and miR-483-5p the most overexpressed (FC = 1.804). miR-483-5p also demonstrated significant overexpression in the pre-operative serum of these patients, with ROC analysis demonstrating an overall predictive accuracy of 78%.

Conclusions

This study provides the first description of atrial myocardial and circulating plasma microRNA in POAF patients. Our findings suggest POAF may be associated with pre-existing atrial substrate differences predisposing to arrhythmogenesis. Moreover, this study highlights the potential for miR-483-5p in biomarker development. Further work must now perform prospective, targeted validation of these results in a larger patient cohort.

SUBMITTER: Harling L 

PROVIDER: S-EPMC5226070 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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Publications

Elevated serum microRNA 483-5p levels may predict patients at risk of post-operative atrial fibrillation.

Harling Leanne L   Lambert Jonathan J   Ashrafian Hutan H   Darzi Ara A   Gooderham Nigel J NJ   Athanasiou Thanos T  

European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery 20160715 1


<h4>Objectives</h4>Post-operative atrial fibrillation (POAF) is the commonest post-operative cardiac arrhythmia, affecting ∼1 in 3 patients undergoing coronary artery bypass grafting (CABG). Although its aetiology is complex, atrial substrate changes may pre-dispose to its onset. This study aims to ascertain the atrial microRNA signature of POAF and determine the potential for circulating microRNA as a pre-operative biomarker for this arrhythmia.<h4>Methods</h4>Thirty-four patients undergoing no  ...[more]

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