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Genetic structure of Mount Huang honey bee (Apis cerana) populations: evidence from microsatellite polymorphism.

ABSTRACT: BACKGROUND:The Mount Huang eastern honey bees (Apis cerana) are an endemic population, which is well adapted to the local agricultural and ecological environment. In this study, the genetic structure of seven eastern honey bees (A. cerana) populations from Mount Huang in China were analyzed by SSR (simple sequence repeat) markers. RESULTS:The results revealed that 16 pairs of primers used amplified a total of 143 alleles. The number of alleles per locus ranged from 6 to 13, with a mean value of 8.94 alleles per locus. Observed and expected heterozygosities showed mean values of 0.446 and 0.831 respectively. UPGMA cluster analysis grouped seven eastern honey bees in three groups. CONCLUSION:The results obtained show a high genetic diversity in the honey bee populations studied in Mount Huang, and high differentiation among all the populations, suggesting that scarce exchange of honey bee species happened in Mount Huang. Our study demonstrated that the Mount Huang honey bee populations still have a natural genome worth being protected for conservation.

SUBMITTER: Liu F

PROVIDER: S-EPMC5226090 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Genetic structure of Mount Huang honey bee (<i>Apis cerana</i>) populations: evidence from microsatellite polymorphism.

Liu Fang F Shi Tengfei T Huang Sisi S Yu Linsheng L Bi Shoudong S

Hereditas 20160706

<h4>Background</h4>The Mount Huang eastern honey bees (<i>Apis cerana</i>) are an endemic population, which is well adapted to the local agricultural and ecological environment. In this study, the genetic structure of seven eastern honey bees (<i>A. cerana</i>) populations from Mount Huang in China were analyzed by SSR (simple sequence repeat) markers.<h4>Results</h4>The results revealed that 16 pairs of primers used amplified a total of 143 alleles. The number of alleles per locus ranged from 6 ...[more]

PMID: 28096770

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Project description:IntroductionThe functional relevance of intra-species diversity in natural microbial communities remains largely unexplored. The guts of two closely related honey bee species, Apis cerana and A. mellifera, are colonised by a similar set of core bacterial species composed of host-specific strains, thereby providing a good model for an intra-species diversity study.ObjectivesWe aim to assess the functional relevance of intra-species diversity of A. cerana and A. mellifera gut microbiota.MethodsHoney bee workers were collected from four regions of China. Their gut microbiomes were investigated by shotgun metagenomic sequencing, and the bacterial compositions were compared at the species level. A cross-species colonisation assay was conducted, with the gut metabolomes being characterised by LC-MS/MS.ResultsComparative analysis showed that the strain composition of the core bacterial species was host-specific. These core bacterial species presented distinctive functional profiles between the hosts. However, the overall functional profiles of the A. cerana and A. mellifera gut microbiomes were similar; this was further supported by the consistency of the honey bees' gut metabolome, as the gut microbiota of different honey bee species showed rather similar metabolic profiles in the cross-species colonisation assay. Moreover, this experiment also demonstrated that the gut microbiota of A. cerana and A. mellifera could cross colonise between the two honey bee species.ConclusionOur findings revealed functional differences in most core gut bacteria between the guts of A. cerana and A. mellifera, which may be associated with their inter-species diversity. However, the functional profiles of the overall gut microbiomes between the two honey bee species converge, probably as a result of the overlapping ecological niches of the two species. Our findings provide critical insights into the evolution and functional roles of the mutualistic microbiota of honey bees and reveal that functional redundancy could stabilise the gene content diversity at the strain-level within the gut community.

Dataset Information

Genetic structure of Mount Huang honey bee (Apis cerana) populations: evidence from microsatellite polymorphism.

Publications

Genetic structure of Mount Huang honey bee (<i>Apis cerana</i>) populations: evidence from microsatellite polymorphism.

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