Dataset Information

Genome sequence of the copper resistant and acid-tolerant Desulfosporosinus sp. BG isolated from the tailings of a molybdenum-tungsten mine in the Transbaikal area.

ABSTRACT: Here, we report on the draft genome of a copper-resistant and acidophilic Desulfosporosinus sp. BG, isolated from the tailings of a molybdenum-tungsten mine in Transbaikal area. The draft genome has a size of 4.52 Mb and encodes transporters of heavy metals. The phylogenetic analysis based on concatenated ribosomal proteins revealed that strain BG clusters together with the other acidophilic copper-resistant strains Desulfosporosinus sp. OT and Desulfosporosinus sp. I2. The K⁺-ATPase, Na⁺/H⁺ antiporter and amino acid decarboxylases may participate in enabling growth at low pH. The draft genome sequence and annotation have been deposited at GenBank under the accession number NZ_MASS00000000.

SUBMITTER: Karnachuk OV

PROVIDER: S-EPMC5228091 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Genome sequence of the copper resistant and acid-tolerant Desulfosporosinus sp. BG isolated from the tailings of a molybdenum-tungsten mine in the Transbaikal area.

Karnachuk Olga V OV Kadnikov Vitalii V VV Panova Inna A IA Mardanov Andrey V AV Beletsky Alexey V AV Danilova Erzhena V EV Avakyan Marat R MR Ravin Nikolai V NV

Genomics data 20161229

Here, we report on the draft genome of a copper-resistant and acidophilic Desulfosporosinus sp. BG, isolated from the tailings of a molybdenum-tungsten mine in Transbaikal area. The draft genome has a size of 4.52 Mb and encodes transporters of heavy metals. The phylogenetic analysis based on concatenated ribosomal proteins revealed that strain BG clusters together with the other acidophilic copper-resistant strains Desulfosporosinus sp. OT and Desulfosporosinus sp. I2. The ...[more]

PMID: 28116241

Similar Datasets

Project description:Copper mining, the central axis of Chile's economic development, produces a large number of tailings, which become a potential environmental risk. This study aims to evaluate the mechanical properties resulting from the making of Portland cement mixtures with tailings as aggregates so that they can be eventually used in paving stones for building inactive tailings dams. Tailings coming from two dams at a concentration plant located in Taltal (Chile) were used. Currently, Dam 1 is inactive, while Dam 2 is active. The tailings samples obtained from both dams were granulometrically characterized by sieving. In addition, pH, humidity, Eh, and mineralogical assays (sulfides, oxides, sulfates, carbonates, phosphates, and silicates) were measured. The fines content of the tailings from Dams 1 and 2 with a sieve size of N°200 ASTM were 76.2% and 29.6%, respectively. Therefore, owing to their high percentage of fines, they cannot be as used as concrete aggregates. Aggregates must contain a maximum percentage of fines so that mortars and concrete can meet Chilean standards. In this paper, to comply with a 7% and 15% fines content lower than 0.075 mm, tailings materials were mixed with conventional aggregates containing very little fines. In addition, a reference mixture was made with only tailings aggregates with and without a superplasticizer additive. To measure the mixtures of cement, aggregates, and tailings, bending and compression strength assays were made of the specimens after a 28-day curing, according to the Chilean standard. The results of the study show that the addition of only part of the tailings to the mixture increases bending strength by 26% and compression strength by 180% compared with the reference mortar, with a fines content lower than 0.075 mm in the 7% mixture, thus allowing paving stone manufacture with tailings materials. In addition, it was possible to increase the workability of the reference mixture by using superplasticizers as additives.

Project description:The oxidation of sulfide-bearing mine tailings catalyzed by acidophilic iron and sulfur-oxidizing bacteria releases toxic metals and other contaminants into soil and groundwater as acid mine drainage. Understanding the environmental variables that control the community structure and metabolic activity of microbes indigenous to tailings (especially the abiotic stressors of low pH and high dissolved metal content) is crucial to developing sustainable bioremediation strategies. We determined the microbial community composition along two continuous vertical gradients of Cu/Ni mine tailings at each of two tailings impoundments near Sudbury, Ontario. 16S rRNA amplicon data showed high variability in community diversity and composition between locations, as well as at different depths within each location. A temporal comparison for one tailings location showed low fluctuation in microbial communities across 2 years. Differences in community composition correlated most strongly with pore-water pH, Eh, alkalinity, salinity, and the concentration of several dissolved metals (including iron, but not copper or nickel). The relative abundances of individual genera differed in their degrees of correlation with geochemical factors. Several abundant lineages present at these locations have not previously been associated with mine tailings environments, including novel species predicted to be involved in iron and sulfur cycling.IMPORTANCEMine tailings represent a significant threat to North American freshwater, with legacy tailings areas generating acid mine drainage (AMD) that contaminates rivers, lakes, and aquifers. Microbial activity accelerates AMD formation through oxidative metabolic processes but may also ameliorate acidic tailings by promoting secondary mineral precipitation and immobilizing dissolved metals. Tailings exhibit high geochemical variation within and between mine sites and may harbor many novel extremophiles adapted to high concentrations of toxic metals. Characterizing the unique microbiomes associated with tailing environments is key to identifying consortia that may be used as the foundation for innovative mine-waste bioremediation strategies. We provide an in-depth analysis of microbial diversity at four copper/nickel mine tailings impoundments, describe how communities (and individual lineages) differ based on geochemical gradients, predict organisms involved in AMD transformations, and identify taxonomically novel groups present that have not previously been observed in mine tailings.

Dataset Information

Genome sequence of the copper resistant and acid-tolerant Desulfosporosinus sp. BG isolated from the tailings of a molybdenum-tungsten mine in the Transbaikal area.

Publications

Genome sequence of the copper resistant and acid-tolerant <i>Desulfosporosinus</i> sp. BG isolated from the tailings of a molybdenum-tungsten mine in the Transbaikal area.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets