Ontology highlight
ABSTRACT:
SUBMITTER: Veschi V
PROVIDER: S-EPMC5233415 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
Veschi Veronica V Liu Zhihui Z Voss Ty C TC Ozbun Laurent L Gryder Berkley B Yan Chunhua C Hu Ying Y Ma Anqi A Jin Jian J Mazur Sharlyn J SJ Lam Norris N Souza Barbara K BK Giannini Giuseppe G Hager Gordon L GL Arrowsmith Cheryl H CH Khan Javed J Appella Ettore E Thiele Carol J CJ
Cancer cell 20170101 1
Given the paucity of druggable mutations in high-risk neuroblastoma (NB), we undertook chromatin-focused small interfering RNA and chemical screens to uncover epigenetic regulators critical for the differentiation block in high-risk NB. High-content Opera imaging identified 53 genes whose loss of expression led to a decrease in NB cell proliferation and 16 also induced differentiation. From these, the secondary chemical screen identified SETD8, the H4<sup>K20me1</sup> methyltransferase, as a dru ...[more]