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Characterization of two polyvalent phages infecting Enterobacteriaceae.

ABSTRACT: Bacteriophages display remarkable genetic diversity and host specificity. In this study, we explore phages infecting bacterial strains of the Enterobacteriaceae family because of their ability to infect related but distinct hosts. We isolated and characterized two novel virulent phages, SH6 and SH7, using a strain of Shigella flexneri as host bacterium. Morphological and genomic analyses revealed that phage SH6 belongs to the T1virus genus of the Siphoviridae family. Conversely, phage SH7 was classified in the T4virus genus of the Myoviridae family. Phage SH6 had a short latent period of 16?min and a burst size of 103?±?16 PFU/infected cell while the phage SH7 latent period was 23?min with a much lower burst size of 26?±?5 PFU/infected cell. Moreover, phage SH6 was sensitive to acidic conditions (pH?

SUBMITTER: Hamdi S

PROVIDER: S-EPMC5238451 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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Characterization of two polyvalent phages infecting Enterobacteriaceae.

Hamdi Sana S Rousseau Geneviève M GM Labrie Simon J SJ Tremblay Denise M DM Kourda Rim Saïed RS Ben Slama Karim K Moineau Sylvain S

Scientific reports 20170116

Bacteriophages display remarkable genetic diversity and host specificity. In this study, we explore phages infecting bacterial strains of the Enterobacteriaceae family because of their ability to infect related but distinct hosts. We isolated and characterized two novel virulent phages, SH6 and SH7, using a strain of Shigella flexneri as host bacterium. Morphological and genomic analyses revealed that phage SH6 belongs to the T1virus genus of the Siphoviridae family. Conversely, phage SH7 was cl ...[more]

PMID: 28091598

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