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High-molecular-weight hyaluronic acid attenuated matrix metalloproteinase-1 and -3 expression via CD44 in tendinopathy.

ABSTRACT: Evidence indicates that hyaluronic acid (HA) mitigates tendinopathy, but the effect of molecular weight is unclear. We investigated the effects of different concentrations and different molecular weights of HA (350?kDa, 1500?kDa, and 3000?kDa) on matrix metalloproteinase (MMP)-1 and -3 expression in IL-1?-stimulated rat tenocytes, and on their dynamic expression in peritendinous effusion from patients with long head of biceps (LHB) tendinopathy after high-molecular-weight (HMW)-HA treatments. Reverse transcription PCR, real-time PCR, and ELISA were used to determine MMP-1 and -3expression. Because CD44 was clearly expressed in the plasma membranes of cultured tenocytes, OX-50, a CD44 antagonist, was used to inhibit CD44 to evaluate the HA mechanism. HA (3000?kDa) significantly (p?

SUBMITTER: Wu PT

PROVIDER: S-EPMC5238506 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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High-molecular-weight hyaluronic acid attenuated matrix metalloproteinase-1 and -3 expression via CD44 in tendinopathy.

Wu Po-Ting PT Kuo Li-Chieh LC Su Fong-Chin FC Chen Shih-Yao SY Hsu Tai-I TI Li Chung-Yi CY Tsai Kuen-Jer KJ Jou I-Ming IM

Scientific reports 20170116

Evidence indicates that hyaluronic acid (HA) mitigates tendinopathy, but the effect of molecular weight is unclear. We investigated the effects of different concentrations and different molecular weights of HA (350 kDa, 1500 kDa, and 3000 kDa) on matrix metalloproteinase (MMP)-1 and -3 expression in IL-1β-stimulated rat tenocytes, and on their dynamic expression in peritendinous effusion from patients with long head of biceps (LHB) tendinopathy after high-molecular-weight (HMW)-HA treatments. Re ...[more]

PMID: 28091588

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