Induced fit of the peptidyl-transferase center of the ribosome and conformational freedom of the esterified amino acids.

ABSTRACT: The catalytic site of most enzymes can efficiently handle only one substrate. In contrast, the ribosome is capable of polymerizing at a similar rate at least 20 different kinds of amino acids from aminoacyl-tRNA carriers while using just one catalytic site, the peptidyl-transferase center (PTC). An induced-fit mechanism has been uncovered in the PTC, but a possible connection between this mechanism and the uniform handling of the substrates has not been investigated. We present an analysis of published ribosome structures supporting the hypothesis that the induced fit eliminates unreactive rotamers predominantly populated for some A-site aminoacyl esters before induction. We show that this hypothesis is fully consistent with the wealth of kinetic data obtained with these substrates. Our an

SUBMITTER: Lehmann J 

PROVIDER: S-EPMC5238797 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature