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An oil-in-water nanoemulsion enhances immunogenicity of H5N1 vaccine in mice.

ABSTRACT: To enhance the immunogenicity of the Influenza H5N1 vaccine, we developed an oil-in-water nanoemulsion (NE) adjuvant. NE displayed good temperature stability and maintained particle size. More importantly, it significantly enhanced IL-6 and MCP-1 production to recruit innate cells, including neutrophils, monocytes/macrophages and dendritic cells to the local environment. Furthermore, NE enhanced dendritic cell function to induce robust antigen-specific T and B cell immune responses. NE-adjuvanted H5N1 vaccine not only elicited significantly higher and long-lasting antibody responses, but also conferred enhanced protection against homologous clade 1 as well as heterologous clade 2 H5N1 virus challenge in young as well as in aged mice. The pre-existing immunity to seasonal influenza did not affect the immunogenicity of NE-adjuvanted H5N1 vaccine.

SUBMITTER: Cao W 

PROVIDER: S-EPMC5240149 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

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An oil-in-water nanoemulsion enhances immunogenicity of H5N1 vaccine in mice.

Cao Weiping W   Davis William G WG   Kim Jin Hyang JH   De La Cruz Juan A JA   Taylor Andrew A   Hendrickson Grant R GR   Kumar Amrita A   Ranjan Priya P   Lyon L Andrew LA   Katz Jacqueline M JM   Gangappa Shivaprakash S   Sambhara Suryaprakash S  

Nanomedicine : nanotechnology, biology, and medicine 20160423 7

To enhance the immunogenicity of the Influenza H5N1 vaccine, we developed an oil-in-water nanoemulsion (NE) adjuvant. NE displayed good temperature stability and maintained particle size. More importantly, it significantly enhanced IL-6 and MCP-1 production to recruit innate cells, including neutrophils, monocytes/macrophages and dendritic cells to the local environment. Furthermore, NE enhanced dendritic cell function to induce robust antigen-specific T and B cell immune responses. NE-adjuvante  ...[more]

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