Ontology highlight
ABSTRACT:
SUBMITTER: Zucchelli C
PROVIDER: S-EPMC5240567 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
Zucchelli Chiara C Ferrari Elena E Blasi Francesco F Musco Giovanna G Bruckmann Chiara C
Scientific reports 20170117
PREP1 and PBX1 are homeodomain (HD) transcription factors that play crucial roles in embryonic development. Here, we present the first biophysical characterization of a PREP1 HD, and the NMR spectroscopic study of its DNA binding pocket. The data show that residues flanking the HD participate in DNA binding. The kinetic parameters for DNA binding of individual PREP1 and PBX1 HDs, and of their combination, show that isolated PREP1 and PBX1 HDs bind to DNA in a cooperative manner. A novel PREP1 mo ...[more]