Ontology highlight
ABSTRACT:
SUBMITTER: Vermeersch JJ
PROVIDER: S-EPMC524305 | biostudies-literature | 2004
REPOSITORIES: biostudies-literature
Vermeersch Jacqueline J JJ Christmann-Franck Serge S Karabashyan Leon V LV Fermandjian Serge S Mirambeau Gilles G Der Garabedian P Arsène PA
Nucleic acids research 20041019 18
The present results demonstrate that pyridoxal, pyridoxal 5'-phosphate (PLP) and pyridoxal 5'-diphospho-5'-adenosine (PLP-AMP) inhibit Candida guilliermondii and human DNA topoisomerases I in forming an aldimine with the epsilon-amino group of an active site lysine. PLP acts as a competitive inhibitor of C.guilliermondii topoisomerase I (K(i) = 40 microM) that blocks the cleavable complex formation. Chemical reduction of PLP-treated enzyme reveals incorporation of 1 mol of PLP per mol of protein ...[more]