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Simultaneous Detection of Dopamine and Uric Acid Using a Poly(l-lysine)/Graphene Oxide Modified Electrode.

ABSTRACT: A novel, simple and selective electrochemical method was investigated for the simultaneous detection of dopamine (DA) and uric acid (UA) on a poly(l-lysine)/graphene oxide (GO) modified glassy carbon electrode (PLL/GO/GCE) by differential pulse voltammetry (DPV). The electrochemically prepared PLL/GO sensory platform toward the oxidation of UA and DA exhibited several advantages, including high effective surface area, more active sites and enhanced electrochemical activity. Compared to the PLL-modified GCE (PLL/GCE), GO-modified GCE and bare GCE, the PLL/GO/GCE exhibited an increase in the anodic potential difference and a remarkable enhancement in the current responses for both UA and DA. For the simultaneous detection of DA and UA, the detection limits of 0.021 and 0.074 ?M were obtained, while 0.031 and 0.018 ?M were obtained as the detection limits for the selective detection of UA and DA, using DPV in the linear concentration ranges of 0.5 to 20.0 and 0.5 to 35 ?M, respectively. In addition, the PLL/GO/GCE demonstrated good reproducibility, long-term stability, excellent selectivity and negligible interference of ascorbic acid (AA). The proposed modified electrode was successfully implemented in the simultaneous detection of DA and UA in human blood serum, urine and dopamine hydrochloride injection with satisfactory results.

SUBMITTER: Zhang Y

PROVIDER: S-EPMC5245187 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature

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Publications

Simultaneous Detection of Dopamine and Uric Acid Using a Poly(l-lysine)/Graphene Oxide Modified Electrode.

Zhang Yuehua Y Lei Wu W Xu Yujuan Y Xia Xifeng X Hao Qingli Q

Nanomaterials (Basel, Switzerland) 20160928 10

A novel, simple and selective electrochemical method was investigated for the simultaneous detection of dopamine (DA) and uric acid (UA) on a poly(l-lysine)/graphene oxide (GO) modified glassy carbon electrode (PLL/GO/GCE) by differential pulse voltammetry (DPV). The electrochemically prepared PLL/GO sensory platform toward the oxidation of UA and DA exhibited several advantages, including high effective surface area, more active sites and enhanced electrochemical activity. Compared to the PLL-m ...[more]

PMID: 28335305

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Project description:Using a simple drop-casting technique, we successfully fabricated a sensitive electrochemical sensor based on the composite of reduced graphene oxide (RGO) and multiwalled carbon nanotubes (MWCNT) deposited on the surface of a glassy carbon electrode (GCE) for individual and simultaneous measurements of hydroquinone (HQ), dopamine (DA), and uric acid (UA). The nanocomposite of RGO/MWCNT was further characterized in terms of its structural properties, surface morphology, and topography using Raman, FT-IR spectroscopy, SEM, HRTEM, and AFM. Then, the proposed sensor for simultaneous measurement of HQ, DA, and UA based on RGO/MWCNT-modified GCE was investigated for its electrochemical behavior and electroanalytical performances using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV). In addition, the composition ratio between RGO and MWCT was 1 : 1 showing the highest electrochemical response for simultaneous detection of HQ, DA, and UA. Owing to the synergistic effect between RGO and MWCNT leading to excellent conductivity properties, the proposed sensor exhibited improved electrochemical response at pH 7 toward the oxidation processes of HQ, DA, and UA on the surface of modified electrode. The proposed sensor demonstrated three well-defined anodic peaks of these analytes with their linear concentrations ranges of 3.0-150.0 μM for HQ, 4.0-100.0 μM for DA, and 2.0-70.0 μM for UA. The limit of detection values for the simultaneous detection of HQ, DA, and UA were found as follows 0.400 ± 0.014, 0.500 ± 0.006, and 0.300 ± 0.016 μM, respectively. The additional features of this proposed sensor are high reproducibility and stability for the simultaneous detection of HQ, DA, and UA with negligible interference effect from interferents such as Mg2+, K+, Cl-, ascorbic acid, and glucose. An acceptable percentage of recovery was also shown by this sensor for simultaneous measurements of HQ, DA, and UA using 6 samples of human urine. In summary, the RGO/MWCNT nanocomposite has been shown to be a promising platform for rapid, simple, and reliable determination of simultaneous measurements of HQ, DA, and UA in practical applications.

Dataset Information

Simultaneous Detection of Dopamine and Uric Acid Using a Poly(l-lysine)/Graphene Oxide Modified Electrode.

Publications

Simultaneous Detection of Dopamine and Uric Acid Using a Poly(l-lysine)/Graphene Oxide Modified Electrode.

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