Dataset Information

CD1^- and CD1⁺ porcine blood dendritic cells are enriched for the orthologues of the two major mammalian conventional subsets.

ABSTRACT: Conventional dendritic cells (cDC) are professional antigen-presenting cells that induce immune activation or tolerance. Two functionally specialised populations, termed cDC1 and cDC2, have been described in humans, mice, ruminants and recently in pigs. Pigs are an important biomedical model species and a key source of animal protein; therefore further understanding of their immune system will help underpin the development of disease prevention strategies. To characterise cDC populations in porcine blood, DC were enriched from PBMC by CD14 depletion and CD172a enrichment then stained with lineage mAbs (Lin; CD3, CD8?, CD14 and CD21) and mAbs specific for CD172a, CD1 and CD4. Two distinct porcine cDC subpopulations were FACSorted CD1^- cDC (Lin^-CD172⁺ CD1^-CD4^-) and CD1⁺ cDC (Lin^-CD172a⁺ CD1⁺ CD4^-), and characterised by phenotypic and functional analyses. CD1⁺ cDC were distinct from CD1^- cDC, expressing higher levels of CD172a, MHC class II and CD11b. Following TLR stimulation, CD1⁺ cDC produced IL-8 and IL-10 while CD1^- cDC secreted IFN-?, IL-12 and TNF-?. CD1^- cDC were superior in stimulating allogeneic T cell responses and in cross-presenting viral antigens to CD8 T cells. Comparison of transcriptional profiles further suggested that the CD1^- and CD1⁺ populations were enriched for the orthologues of cDC1 and cDC2 subsets respectively.

SUBMITTER: Edwards JC

PROVIDER: S-EPMC5247722 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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CD1<sup>-</sup> and CD1<sup>+</sup> porcine blood dendritic cells are enriched for the orthologues of the two major mammalian conventional subsets.

Edwards Jane C JC Everett Helen E HE Pedrera Miriam M Mokhtar Helen H Marchi Emanuele E Soldevila Ferran F Kaveh Daryan A DA Hogarth Philip J PJ Johns Helen L HL Nunez-Garcia Javier J Steinbach Falko F Crooke Helen R HR Graham Simon P SP

Scientific reports 20170120

Conventional dendritic cells (cDC) are professional antigen-presenting cells that induce immune activation or tolerance. Two functionally specialised populations, termed cDC1 and cDC2, have been described in humans, mice, ruminants and recently in pigs. Pigs are an important biomedical model species and a key source of animal protein; therefore further understanding of their immune system will help underpin the development of disease prevention strategies. To characterise cDC populations in porc ...[more]

PMID: 28106145

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Project description:HIV-2 infection is characterized by low viremia and slow disease progression as compared to HIV-1 infection. Circulating CD14++CD16+ monocytes were found to accumulate and CD11c+ conventional dendritic cells (cDC) to be depleted in a Portuguese cohort of people living with HIV-2 (PLWHIV-2), compared to blood bank healthy donors (HD). We studied more precisely classical monocytes; CD16+ inflammatory (intermediate, non-classical and slan+ monocytes, known to accumulate during viremic HIV-1 infection); cDC1, important for cross-presentation, and cDC2, both depleted during HIV-1 infection. We analyzed by flow cytometry these PBMC subsets from Paris area residents: 29 asymptomatic, untreated PLWHIV-2 from the IMMUNOVIR-2 study, part of the ANRS-CO5 HIV-2 cohort: 19 long-term non-progressors (LTNP; infection ≥8 years, undetectable viral load, stable CD4 counts≥500/μL; 17 of West-African origin -WA), and 10 non-LTNP (P; progressive infection; 9 WA); and 30 age-and sex-matched controls: 16 blood bank HD with unknown geographical origin, and 10 HD of WA origin (GeoHD). We measured plasma bacterial translocation markers by ELISA. Non-classical monocyte counts were higher in GeoHD than in HD (54 vs. 32 cells/μL, p = 0.0002). Slan+ monocyte counts were twice as high in GeoHD than in HD (WA: 28 vs. 13 cells/μL, p = 0.0002). Thus cell counts were compared only between participants of WA origin. They were similar in LTNP, P and GeoHD, indicating that there were no HIV-2 related differences. cDC counts did not show major differences between the groups. Interestingly, inflammatory monocyte counts correlated with plasma sCD14 and LBP only in PLWHIV-2, especially LTNP, and not in GeoHD. In conclusion, in LTNP PLWHIV-2, inflammatory monocyte counts correlated with LBP or sCD14 plasma levels, indicating a potential innate immune response to subclinical bacterial translocation. As GeoHD had higher inflammatory monocyte counts than HD, our data also show that specific controls are important to refine innate immunity studies.

Dataset Information

CD1^- and CD1⁺ porcine blood dendritic cells are enriched for the orthologues of the two major mammalian conventional subsets.

Publications

CD1<sup>-</sup> and CD1<sup>+</sup> porcine blood dendritic cells are enriched for the orthologues of the two major mammalian conventional subsets.

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OmicsDI is part of the ELIXIR infrastructure

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Dataset Information

CD1- and CD1+ porcine blood dendritic cells are enriched for the orthologues of the two major mammalian conventional subsets.

Publications

CD1<sup>-</sup> and CD1<sup>+</sup> porcine blood dendritic cells are enriched for the orthologues of the two major mammalian conventional subsets.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

CD1^- and CD1⁺ porcine blood dendritic cells are enriched for the orthologues of the two major mammalian conventional subsets.