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Identification of LukPQ, a novel, equid-adapted leukocidin of Staphylococcus aureus.


ABSTRACT: Bicomponent pore-forming leukocidins are a family of potent toxins secreted by Staphylococcus aureus, which target white blood cells preferentially and consist of an S- and an F-component. The S-component recognizes a receptor on the host cell, enabling high-affinity binding to the cell surface, after which the toxins form a pore that penetrates the cell lipid bilayer. Until now, six different leukocidins have been described, some of which are host and cell specific. Here, we identify and characterise a novel S. aureus leukocidin; LukPQ. LukPQ is encoded on a 45?kb prophage (?Saeq1) found in six different clonal lineages, almost exclusively in strains cultured from equids. We show that LukPQ is a potent and specific killer of equine neutrophils and identify equine-CXCRA and CXCR2 as its target receptors. Although the S-component (LukP) is highly similar to the S-component of LukED, the species specificity of LukPQ and LukED differs. By forming non-canonical toxin pairs, we identify that the F-component contributes to the observed host tropism of LukPQ, thereby challenging the current paradigm that leukocidin specificity is driven solely by the S-component.

SUBMITTER: Koop G 

PROVIDER: S-EPMC5247767 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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Identification of LukPQ, a novel, equid-adapted leukocidin of Staphylococcus aureus.

Koop Gerrit G   Vrieling Manouk M   Storisteanu Daniel M L DM   Lok Laurence S C LS   Monie Tom T   van Wigcheren Glenn G   Raisen Claire C   Ba Xiaoliang X   Gleadall Nicholas N   Hadjirin Nazreen N   Timmerman Arjen J AJ   Wagenaar Jaap A JA   Klunder Heleen M HM   Fitzgerald J Ross JR   Zadoks Ruth R   Paterson Gavin K GK   Torres Carmen C   Waller Andrew S AS   Loeffler Anette A   Loncaric Igor I   Hoet Armando E AE   Bergström Karin K   De Martino Luisa L   Pomba Constança C   de Lencastre Hermínia H   Ben Slama Karim K   Gharsa Haythem H   Richardson Emily J EJ   Chilvers Edwin R ER   de Haas Carla C   van Kessel Kok K   van Strijp Jos A G JA   Harrison Ewan M EM   Holmes Mark A MA  

Scientific reports 20170120


Bicomponent pore-forming leukocidins are a family of potent toxins secreted by Staphylococcus aureus, which target white blood cells preferentially and consist of an S- and an F-component. The S-component recognizes a receptor on the host cell, enabling high-affinity binding to the cell surface, after which the toxins form a pore that penetrates the cell lipid bilayer. Until now, six different leukocidins have been described, some of which are host and cell specific. Here, we identify and charac  ...[more]

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