Dataset Information

T-6b allocates more assimilation product for oil synthesis and less for polysaccharide synthesis during the seed development of Arabidopsis thaliana.

ABSTRACT: BACKGROUND:As an Agrobacterium tumefaciens T-DNA oncogene, T-6b induces the development of tumors and the enation syndrome in vegetative tissues of transgenic plants. Most of these effects are related to increases in soluble sugar contents. To verify the potential roles of T-6b in the distribution of carbon in developing seeds, not in vegetative tissues, we fused an endosperm-specific promoter to the T-6b gene for expression in transgenic Arabidopsis thaliana plants. RESULTS:The expression of T-6b in reproductive organs did not induce the development of the enation syndrome, and moreover, promoted endosperm expansion, which increased the total seed biomass by more than 10%. Additionally, T-6b also increased oil content in mature seeds by more than 10% accompanied with the decrease of starch and mucilage content at the same time. CONCLUSIONS:T-6b enhances seed biomass and helps oil biosynthesis but not polysaccharides in reproductive organs without disturbing vegetative growth and development. Our findings suggest T-6b may be very useful for increasing oil production in biodiesel plants.

SUBMITTER: Jin Y

PROVIDER: S-EPMC5251281 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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T-6b allocates more assimilation product for oil synthesis and less for polysaccharide synthesis during the seed development of Arabidopsis thaliana.

Jin Yunkai Y Hu Jia J Liu Xun X Ruan Ying Y Sun Chuanxin C Liu Chunlin C

Biotechnology for biofuels 20170121

<h4>Background</h4>As an Agrobacterium tumefaciens T-DNA oncogene, T-6b induces the development of tumors and the enation syndrome in vegetative tissues of transgenic plants. Most of these effects are related to increases in soluble sugar contents. To verify the potential roles of T-6b in the distribution of carbon in developing seeds, not in vegetative tissues, we fused an endosperm-specific promoter to the T-6b gene for expression in transgenic Arabidopsis thaliana ...[more]

PMID: 28127400

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Project description:Photosynthetic assimilation is remarkably altered by heat and drought, and this depends on the individual or combined occurrence of stressors and their respective intensities and durations. Abiotic stressors may also alter the nutritional quality and economic value of crops. In this controlled greenhouse study, we evaluated the response of Brassica napus L., from flowering to seed development, to two temperature and water treatments and a combination of these treatments. The diffusional limitations of stomatal conductance and mesophyll conductance on photosynthesis, as well as resource-use efficiency (particularly water and nitrogen), were assessed. In addition, the effects of stressors on the seed fatty acid content and composition and the total protein content were examined. The results showed that the reduction in the net photosynthetic assimilation rate was caused by combinations of heat and drought (heat + drought) treatments, by drought alone, and, to a lesser extent, by heat alone. The stomatal conductance decreased under drought and heat + drought treatments but not under heat. Conversely, the mesophyll conductance was reduced significantly in the plants exposed to heat and heat + drought but not in the plants exposed to drought alone. The carboxylation efficiency rate and the electron transport rate were reduced under the heat treatment. The seed yield was reduced by 85.3% under the heat treatment and, to a lesser extent, under the drought treatment (31%). This emphasizes the devastating effects of hotter weather on seed formation and development. Seed oil content decreased by 52% in the plants exposed to heat, the protein content increased under all the stress treatments. Heat treatment had a more deleterious effect than drought on the seed oil composition, leading to enhanced levels of saturated fatty oils and, consequently, desaturation efficiency, a measure of oil frying ability. Overall, this study showed that except for the photosynthetic assimilation rate and stomatal conductance, heat, rather than drought, negatively affected the photosynthetic capacity, yield, and oil quality attributes when imposed during the flowering and silique-filling stages. This result highlights the necessity for a better understanding of heat tolerance mechanisms in crops to help to create germplasms that are adapted to rapid climate warming.

Dataset Information

T-6b allocates more assimilation product for oil synthesis and less for polysaccharide synthesis during the seed development of Arabidopsis thaliana.

Publications

T-<i>6b</i> allocates more assimilation product for oil synthesis and less for polysaccharide synthesis during the seed development of <i>Arabidopsis thaliana</i>.

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