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Multiplexed detection of pathogen-specific DNA using engineered zinc finger proteins without target amplification.

ABSTRACT: Multiplexed detection of pathogen-specific DNA sequences in a simple and reliable way is in great demand for clinical and biomedical applications. However, there is still a lack of available DNA detection methods that are simple and pathogen-selective for point-of-care (POC) testing. Here, we report a novel zinc finger protein (ZFP)-based chemiluminescent method for direct detection of pathogenic double-stranded DNA (dsDNA) in a multiplexed platform. ZFPs are custom-designed to identify unique pathogenic DNA sequences. ZFP-based chemiluminescent detection of dsDNA provides sufficient sensitivity (?50 fmol) and high specificity without target DNA amplification. Our study addresses the potential of developing a simple and selective pathogen detection method in a multiplexed fashion needed for POC application.

SUBMITTER: Kim MS

PROVIDER: S-EPMC5258119 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

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Multiplexed detection of pathogen-specific DNA using engineered zinc finger proteins without target amplification.

Kim Moon-Soo MS Kim Juhwa J

Analytical methods : advancing methods and applications 20160816 37

Multiplexed detection of pathogen-specific DNA sequences in a simple and reliable way is in great demand for clinical and biomedical applications. However, there is still a lack of available DNA detection methods that are simple and pathogen-selective for point-of-care (POC) testing. Here, we report a novel zinc finger protein (ZFP)-based chemiluminescent method for direct detection of pathogenic double-stranded DNA (dsDNA) in a multiplexed platform. ZFPs are custom-designed to identify unique p ...[more]

PMID: 28127406

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