Ontology highlight
ABSTRACT:
SUBMITTER: Erlendsson S
PROVIDER: S-EPMC5262378 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
Erlendsson Simon S Gotfryd Kamil K Larsen Flemming Hofmann FH Mortensen Jonas Sigurd JS Geiger Michel-Andreas MA van Rossum Barth-Jan BJ Oschkinat Hartmut H Gether Ulrik U Teilum Kaare K Loland Claus J CJ
eLife 20170124
The Neurotransmitter:Sodium Symporters (NSSs) represent an important class of proteins mediating sodium-dependent uptake of neurotransmitters from the extracellular space. The substrate binding stoichiometry of the bacterial NSS protein, LeuT, and thus the principal transport mechanism, has been heavily debated. Here we used solid state NMR to specifically characterize the bound leucine ligand and probe the number of binding sites in LeuT. We were able to produce high-quality NMR spectra of subs ...[more]