Ontology highlight
ABSTRACT:
SUBMITTER: Funke JJ
PROVIDER: S-EPMC5262459 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
Funke Jonas J JJ Ketterer Philip P Lieleg Corinna C Schunter Sarah S Korber Philipp P Dietz Hendrik H
Science advances 20161123 11
Revealing the energy landscape for nucleosome association may contribute to the understanding of higher-order chromatin structures and their impact on genome regulation. We accomplish this in a direct measurement by integrating two nucleosomes into a DNA origami-based force spectrometer, which enabled subnanometer-resolution measurements of nucleosome-nucleosome distance frequencies via single-particle electron microscopy imaging. From the data, we derived the Boltzmann-weighted distance-depende ...[more]