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Engineering Escherichia coli for light-activated cytolysis of mammalian cells.

ABSTRACT: By delivering payloads in response to specific exogenous stimuli, smart bacterial therapeutics have the potential to overcome many limitations of conventional therapies, including poor targeting specificity and dosage control in current cancer treatments. Although not yet explored as a trigger for bacterial drug delivery, light is an ideal induction mechanism because it offers fine spatiotemporal control and is easily and safely administered. Using recent advances in optogenetics, we have engineered two strains of Escherichia coli to secrete a potent mammalian cytotoxin in response to blue or red light. The tools in this study demonstrate the initial feasibility of light-activated bacterial therapeutics for applications such as tumor cytolysis, and their modular nature should enable simple substitution of other payloads of interest.

SUBMITTER: Magaraci MS

PROVIDER: S-EPMC5264543 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature

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Engineering Escherichia coli for light-activated cytolysis of mammalian cells.

Magaraci Michael S MS Veerakumar Avin A Qiao Peter P Amurthur Ashwin A Lee Justin Y JY Miller Jordan S JS Goulian Mark M Sarkar Casim A CA

ACS synthetic biology 20140114 12

By delivering payloads in response to specific exogenous stimuli, smart bacterial therapeutics have the potential to overcome many limitations of conventional therapies, including poor targeting specificity and dosage control in current cancer treatments. Although not yet explored as a trigger for bacterial drug delivery, light is an ideal induction mechanism because it offers fine spatiotemporal control and is easily and safely administered. Using recent advances in optogenetics, we have engine ...[more]

PMID: 24933444

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