Dataset Information

Comparative analysis of the volatile metabolomes of Pseudomonas aeruginosa clinical isolates.

ABSTRACT: Pseudomonas aeruginosa is a nearly ubiquitous Gram-negative organism, well known to occupy a multitude of environmental niches and cause human infections at a variety of bodily sites, due to its metabolic flexibility, secondary to extensive genetic heterogeneity at the species level. Because of its dynamic metabolism and clinical importance, we sought to perform a comparative analysis on the volatile metabolome (the 'volatilome') produced by P. aeruginosa clinical isolates. In this study, we analyzed the headspace volatile molecules of 24 P. aeruginosa clinical isolates grown in vitro, using 2D gas chromatography time-of-flight mass spectrometry (GC??×??GC-TOFMS). We identified 391 non-redundant compounds that we associate with the growth and metabolism of P. aeruginosa (the 'pan-volatilome'). Of these, 70 were produced by all 24 isolates (the 'core volatilome'), 52 by only a single isolate, and the remaining 269 volatile molecules by a subset. Sixty-five of the detected compounds could be assigned putative compound identifications, of which 43 had not previously been associated with P. aeruginosa. Using the accessory volatile molecules, we determined the inter-strain variation in the metabolomes of these isolates, clustering strains by their metabotypes. Assessing the extent of metabolomic diversity in P. aeruginosa through an analysis of the volatile molecules that it produces is a critical next step in the identification of novel diagnostic or prognostic biomarkers.

SUBMITTER: Bean HD

PROVIDER: S-EPMC5266606 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature

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Comparative analysis of the volatile metabolomes of Pseudomonas aeruginosa clinical isolates.

Bean Heather D HD Rees Christiaan A CA Hill Jane E JE

Journal of breath research 20161121 4

Pseudomonas aeruginosa is a nearly ubiquitous Gram-negative organism, well known to occupy a multitude of environmental niches and cause human infections at a variety of bodily sites, due to its metabolic flexibility, secondary to extensive genetic heterogeneity at the species level. Because of its dynamic metabolism and clinical importance, we sought to perform a comparative analysis on the volatile metabolome (the 'volatilome') produced by P. aeruginosa clinical isolates. In this study, we ana ...[more]

PMID: 27869104

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Project description:Pseudomonas aeruginosa is one of the first causes of acute nosocomial and chronic infections in patients with underlying respiratory pathologies such as cystic fibrosis (CF). It has been proposed that P. aeruginosa accumulates mutations driving to peptidoglycan modifications throughout the development of the CF-associated infection, as a strategy to lower the immune detection hence ameliorating the chronic persistence. As well, some studies dealing with peptidoglycan modifications driving to a better survival within the host have been published in other gram-negatives. According to these facts, the gram-negative peptidoglycan could be considered as a pathogen-associated molecular pattern with very important implications regarding the host's detection-response, worthy to dissect in detail. For this reason, in this work we characterized for the first time the peptidoglycans of three large collections [early CF, late CF and acute infection (bloodstream) P. aeruginosa strains] from qualitative (HPLC), quantitative and inflammatory capacity-related perspectives. The final goal was to identify composition trends potentially supporting the cited strategy of evasion/resistance to the immune system and providing information regarding the differential intrinsic adaptation depending on the type of infection. Although we found several punctual strain-specific particularities, our results indicated a high degree of inter-collection uniformity in the peptidoglycan-related features and the absence of trends amongst the strains studied here. These results suggest that the peptidoglycan of P. aeruginosa is a notably conserved structure in natural isolates regardless of transitory changes that some external conditions could force. Finally, the inverse correlation between the relative amount of stem pentapeptides within the murein sacculus and the resistance to immune lytic attacks against the peptidoglycan was also suggested by our results. Altogether, this work is a major step ahead to understand the biology of peptidoglycan from P. aeruginosa natural strains, hopefully useful in future for therapeutic alternatives design.

Dataset Information

Comparative analysis of the volatile metabolomes of Pseudomonas aeruginosa clinical isolates.

Publications

Comparative analysis of the volatile metabolomes of Pseudomonas aeruginosa clinical isolates.

Similar Datasets

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