Dataset Information

Cultivated Sub-Populations of Soil Microbiomes Retain Early Flowering Plant Trait.

ABSTRACT: The collection of microorganisms found in the root zone of soil, termed the rhizosphere microbiome, has been shown to impact plant growth and development. Here, we tease apart the function of the cultivable portion of the microbiome from the whole microbiome in retaining plant traits modified through artificial selection on flowering time. Specifically, the whole microbiome associated with earlier flowering time of Arabidopsis thaliana was cultivated on four types of solid media to create cultivated fractions of the microbiome. These cultivated microbiomes were subsequently preserved in glycerol, frozen, and revived to yield a portion of the cultivable fraction to compare (1) whole microbiome, (2) cultivable microbiome, and (3) revived, cultivable microbiome controls on early flowering time. Plants grown in soils inoculated with bacteria grown on 25 % Luria broth and 10 % tryptic soy agar retained the early flowering trait. An increase in leaf biomass with two of the cultivated microbiomes (49.4 and 38.5 %) contrasted the lowered biomass effect of the whole microbiome. Inoculation with the cultivated microbiomes that were cryopreserved in glycerol showed no effect on flowering time or leaf biomass. The results indicate that the cultivable portion of a plant's microbiome retains the early flowering effect in A. thaliana, but cryopreservation of the cultivated microbiomes disrupts the microbial effects on flowering time. Furthermore, the contrasting effects on leaf biomass (an indirect response from selection on early flowering time), seen with the whole microbiome versus the cultivable portion, suggests versatility in using cultivation methods to modify multiple traits of plants.

SUBMITTER: Panke-Buisse K

PROVIDER: S-EPMC5272889 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature

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Cultivated Sub-Populations of Soil Microbiomes Retain Early Flowering Plant Trait.

Panke-Buisse Kevin K Lee Stacey S Kao-Kniffin Jenny J

Microbial ecology 20160921 2

The collection of microorganisms found in the root zone of soil, termed the rhizosphere microbiome, has been shown to impact plant growth and development. Here, we tease apart the function of the cultivable portion of the microbiome from the whole microbiome in retaining plant traits modified through artificial selection on flowering time. Specifically, the whole microbiome associated with earlier flowering time of Arabidopsis thaliana was cultivated on four types of solid media to create cultiv ...[more]

PMID: 27655524

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Project description:Soil compaction of arable land, caused by heavy machinery constitutes a major threat to agricultural soils in industrialized countries. The degradation of soil structure due to compaction leads to decreased (macro-) porosity resulting in increased mechanical impedance, which adversely affects root growth and crop productivity. New crop cultivars, with root systems that are adapted to conditions of increased soil strength, are needed to overcome the limiting effects of soil compaction on plant growth. This study aimed (i) to quantify the genetic diversity of early root system development in wheat and to relate this to shoot development under different soil bulk densities and (ii) to test whether root numbers are suitable traits to assess the genotypic tolerance to soil compaction. Fourteen wheat genotypes were grown for 3 weeks in a growth chamber under low (1.3 g cm-3), moderate (1.45 g cm-3), and high soil bulk density (1.6 g cm-3). Using X-ray computed tomography root system development was quantified in weekly intervals, which was complemented by weekly measurements of plant height. The development of the root system, quantified via the number of axial and lateral roots was strongly correlated (0.78 < r < 0.88, p < 0.01) to the development of plant height. Furthermore, significant effects (p < 0.01) of the genotype on root system development and plant vigor traits were observed. Under moderate soil strength final axial and lateral root numbers were significantly correlated (0.57 < r < 0.84, p < 0.05) to shoot dry weight. Furthermore, broad-sense heritability of axial and lateral root number was higher than 50% and comparable to values calculated for shoot traits. Our results showed that there is genetic diversity in wheat with respect to root system responses to increased soil strength and that root numbers are suitable indicators to explain the responses and the tolerance to such conditions. Since root numbers are heritable and can be assessed at high throughput rates under laboratory and field conditions, root number is considered a promising trait for screening toward compaction tolerant varieties.

Dataset Information

Cultivated Sub-Populations of Soil Microbiomes Retain Early Flowering Plant Trait.

Publications

Cultivated Sub-Populations of Soil Microbiomes Retain Early Flowering Plant Trait.

Similar Datasets

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