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Comparative Phosphoproteomic Analysis under High-Nitrogen Fertilizer Reveals Central Phosphoproteins Promoting Wheat Grain Starch and Protein Synthesis.

ABSTRACT: Nitrogen (N) is a macronutrient important for plant growth and development. It also strongly influences starch and protein synthesis, closely related to grain yield and quality. We performed the first comparative phosphoproteomic analysis of developing wheat grains in response to high-N fertilizer. Physiological and biochemical analyses showed that application of high-N fertilizer resulted in significant increases in leaf length and area, chlorophyll content, the activity of key enzymes in leaves such as nitrate reductase (NR), and in grains such as sucrose phosphate synthase (SPS), sucrose synthase (SuSy), and ADP glucose pyrophosphorylase (AGPase). This enhanced enzyme activity led to significant improvements in starch content, grain yield, and ultimately, bread making quality. Comparative phosphoproteomic analysis of developing grains under the application of high-N fertilizer performed 15 and 25 days post-anthesis identified 2470 phosphosites among 1372 phosphoproteins, of which 411 unique proteins displayed significant changes in phosphorylation level (>2-fold or <0.5-fold). These phosphoproteins are involved mainly in signaling transduction, starch synthesis, energy metabolism. Pro-Q diamond staining and Western blotting confirmed our phosphoproteomic results. We propose a putative pathway to elucidate the important roles of the central phosphoproteins regulating grain starch and protein synthesis. Our results provide new insights into the molecular mechanisms of protein phosphorylation modifications involved in grain development, yield and quality formation.

SUBMITTER: Zhen S

PROVIDER: S-EPMC5277015 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Comparative Phosphoproteomic Analysis under High-Nitrogen Fertilizer Reveals Central Phosphoproteins Promoting Wheat Grain Starch and Protein Synthesis.

Zhen Shoumin S Deng Xiong X Zhang Ming M Zhu Gengrui G Lv Dongwen D Lv Dongwen D Wang Yaping Y Zhu Dong D Yan Yueming Y

Frontiers in plant science 20170130

Nitrogen (N) is a macronutrient important for plant growth and development. It also strongly influences starch and protein synthesis, closely related to grain yield and quality. We performed the first comparative phosphoproteomic analysis of developing wheat grains in response to high-N fertilizer. Physiological and biochemical analyses showed that application of high-N fertilizer resulted in significant increases in leaf length and area, chlorophyll content, the activity of key enzymes in leave ...[more]

PMID: 28194157

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Project description:Monitoring nitrogen utilization is crucial in agricultural practices, emphasizing the interrelationship between soil health, nutrient management, and human health. The study was conducted to evaluate the impact of N fertilizer on the nutritional characteristics of diverse S. bicolor varieties, namely Alföldi 1, ES Föehn (Lidea Seeds) with a red pericarp, ES Albanus, Albita, and Farmsugro 180 (all white varieties), the study was conducted in sorghum-producing areas where the crop is non-native. Specifically, the study investigated two soil types: loam clay and sandy soil. Furthermore, the respective varieties were grown under N (27% N CAN) fertilizer conditions, involving 60 kg/ha-1 and 120 kg/ha-1 of the treatment rates applied at each experiment site. We measured the specific element concentration in each sample using the Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES) technology. Certainly, the results demonstrated that the different S. bicolor varieties had unique nutritional characteristics attributed to several factors such as soil type, variety, and treatment, which showed a significance value of (P < 0.05). The findings demonstrated that the treatments had distinct impacts as stimulators and inhibitors for certain elements. Specifically, the application of 120 kg/ha-1 negatively affected the levels of particular elements, such as Ca mg/kg-1, in loam clay and sandy soil. The statistical analysis of trace microelement variance did not show a significance value (P > 0.05) when considering the year factor, which supported the data analysis's reliability and accuracy. In summary, to enhance the nutritional value of sorghum grain and supply nutrient-rich food choices for individuals, consider factors such as fertilizer response, nutrient uptake by grain, element mineral accumulation, and advisory variety. Additional research could enhance the nutritional properties of sorghum to provide the required dietary stuff, such as grain processing, which can render sorghum a proper addition to a healthy and balanced human diet.

Dataset Information

Comparative Phosphoproteomic Analysis under High-Nitrogen Fertilizer Reveals Central Phosphoproteins Promoting Wheat Grain Starch and Protein Synthesis.

Publications

Comparative Phosphoproteomic Analysis under High-Nitrogen Fertilizer Reveals Central Phosphoproteins Promoting Wheat Grain Starch and Protein Synthesis.

Similar Datasets

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