Mitochondrial Ferritin Deletion Exacerbates ?-Amyloid-Induced Neurotoxicity in Mice.

ABSTRACT: Mitochondrial ferritin (FtMt) is a mitochondrial iron storage protein which protects mitochondria from iron-induced oxidative damage. Our previous studies indicate that FtMt attenuates ?-amyloid- and 6-hydroxydopamine-induced neurotoxicity in SH-SY5Y cells. To explore the protective effects of FtMt on ?-amyloid-induced memory impairment and neuronal apoptosis and the mechanisms involved, 10-month-old wild-type and Ftmt knockout mice were infused intracerebroventricularly (ICV) with A?_25-35 to establish an Alzheimer's disease model. Knockout of Ftmt significantly exacerbated A?_25-35-induced learning and memory impairment. The Bcl-2/Bax ratio in mouse hippocampi was decreased and the levels of cleaved caspase-3 and PARP were increased. The number of neuronal cells undergoing apoptosis in the hippocampus was also increased in Ftmt knockout mice. In addition, the levels of L-ferritin and FPN1 in the hippocampus were raised, and the expression of TfR1 was decreased. Increased MDA levels were also detected in Ftmt knockout mice treated with A?_25-35. In conclusion, this study demonstrated that the neurological impairment induced by A?_25-35 was exacerbated in Ftmt knockout mice and that this may relate to increased levels of oxidative stress.

SUBMITTER: Wang P 

PROVIDER: S-EPMC5278219 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature