Ontology highlight
ABSTRACT:
SUBMITTER: Yang H
PROVIDER: S-EPMC5278635 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
Yang Hui H Gao Pu P Rajashankar Kanagalaghatta R KR Patel Dinshaw J DJ
Cell 20161201 7
C2c1 is a newly identified guide RNA-mediated type V-B CRISPR-Cas endonuclease that site-specifically targets and cleaves both strands of target DNA. We have determined crystal structures of Alicyclobacillus acidoterrestris C2c1 (AacC2c1) bound to sgRNA as a binary complex and to target DNAs as ternary complexes, thereby capturing catalytically competent conformations of AacC2c1 with both target and non-target DNA strands independently positioned within a single RuvC catalytic pocket. Moreover, ...[more]