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Single ?-particle irradiation permits real-time visualization of RNF8 accumulation at DNA damaged sites.

ABSTRACT: As well as being a significant source of environmental radiation exposure, ?-particles are increasingly considered for use in targeted radiation therapy. A better understanding of ?-particle induced damage at the DNA scale can be achieved by following their tracks in real-time in targeted living cells. Focused ?-particle microbeams can facilitate this but, due to their low energy (up to a few MeV) and limited range, ?-particles detection, delivery, and follow-up observations of radiation-induced damage remain difficult. In this study, we developed a thin Boron-doped Nano-Crystalline Diamond membrane that allows reliable single ?-particles detection and single cell irradiation with negligible beam scattering. The radiation-induced responses of single 3?MeV ?-particles delivered with focused microbeam are visualized in situ over thirty minutes after irradiation by the accumulation of the GFP-tagged RNF8 protein at DNA damaged sites.

SUBMITTER: Muggiolu G 

PROVIDER: S-EPMC5282495 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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Single α-particle irradiation permits real-time visualization of RNF8 accumulation at DNA damaged sites.

Muggiolu Giovanna G   Pomorski Michal M   Claverie Gérard G   Berthet Guillaume G   Mer-Calfati Christine C   Saada Samuel S   Devès Guillaume G   Simon Marina M   Seznec Hervé H   Barberet Philippe P  

Scientific reports 20170131

As well as being a significant source of environmental radiation exposure, α-particles are increasingly considered for use in targeted radiation therapy. A better understanding of α-particle induced damage at the DNA scale can be achieved by following their tracks in real-time in targeted living cells. Focused α-particle microbeams can facilitate this but, due to their low energy (up to a few MeV) and limited range, α-particles detection, delivery, and follow-up observations of radiation-induced  ...[more]

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