Honeybee locomotion is impaired by Am-Ca_V3 low voltage-activated Ca²⁺ channel antagonist.

ABSTRACT: Voltage-gated Ca²⁺ channels are key transducers of cellular excitability and participate in several crucial physiological responses. In vertebrates, 10 Ca²⁺ channel genes, grouped in 3 families (Ca_V1, Ca_V2 and Ca_V3), have been described and characterized. Insects possess only one member of each family. These genes have been isolated in a limited number of species and very few have been characterized although, in addition to their crucial role, they may represent a collateral target for neurotoxic insecticides. We have isolated the 3 genes coding for the 3 Ca²⁺ channels expressed in Apis mellifera. This work provides the first detailed characterization of the honeybee T-type Ca_V3 Ca²⁺ channel and demonstrates the low toxicity of inhibiting this channel. Comparing Ca²⁺ currents recorded in bee neurons and myocytes with Ca²⁺ currents recorded in Xenopus oocytes expressing the honeybee Ca_V3 gene suggests native expression in bee muscle cells only. High-voltage activated Ca²⁺ channels could be recorded in the somata of different cultured bee neurons. These functional data were confirmed by in situ hybridization, immunolocalization and in vivo analysis of the effects of a Ca_V3 inhibitor. The biophysical and pharmacological characterization and the tissue distribution of Ca_V3 suggest a role in honeybee muscle function.

SUBMITTER: Rousset M 

PROVIDER: S-EPMC5286435 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature