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Construction of a guide-RNA for site-directed RNA mutagenesis utilising intracellular A-to-I RNA editing.


ABSTRACT: As an alternative to DNA mutagenesis, RNA mutagenesis can potentially become a powerful gene-regulation method for fundamental research and applied life sciences. Adenosine-to-inosine (A-to-I) RNA editing alters genetic information at the transcript level and is an important biological process that is commonly conserved in metazoans. Therefore, a versatile RNA-mutagenesis method can be achieved by utilising the intracellular RNA-editing mechanism. Here, we report novel guide RNAs capable of inducing A-to-I mutations by guiding the editing enzyme, human adenosine deaminase acting on RNA (ADAR). These guide RNAs successfully introduced A-to-I mutations into the target-site, which was determined by the reprogrammable antisense region. In ADAR2-over expressing cells, site-directed RNA editing could also be performed by simply introducing the guide RNA. Our guide RNA framework provides basic insights into establishing a generally applicable RNA-mutagenesis method.

SUBMITTER: Fukuda M 

PROVIDER: S-EPMC5288656 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature

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Construction of a guide-RNA for site-directed RNA mutagenesis utilising intracellular A-to-I RNA editing.

Fukuda Masatora M   Umeno Hiromitsu H   Nose Kanako K   Nishitarumizu Azusa A   Noguchi Ryoma R   Nakagawa Hiroyuki H  

Scientific reports 20170202


As an alternative to DNA mutagenesis, RNA mutagenesis can potentially become a powerful gene-regulation method for fundamental research and applied life sciences. Adenosine-to-inosine (A-to-I) RNA editing alters genetic information at the transcript level and is an important biological process that is commonly conserved in metazoans. Therefore, a versatile RNA-mutagenesis method can be achieved by utilising the intracellular RNA-editing mechanism. Here, we report novel guide RNAs capable of indu  ...[more]

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