Ontology highlight
ABSTRACT:
SUBMITTER: Mowry TM
PROVIDER: S-EPMC528885 | biostudies-literature | 2004
REPOSITORIES: biostudies-literature
Mowry Thomas M TM Barbour James D JD
Journal of insect science (Online) 20040723
A molecular procedure incorporating polymerase chain reaction (PCR) of the COI gene and restriction endonuclease digestion of PCR products was used to distinguish Peristenus howardi (Hymenoptera: Braconidae) from four other Peristenus species. Non-solvent extraction of parasite DNA using a commercially available kit proved to be very effective in producing amplifiable template. Use of SfcI endonuclease produced restriction fragments with banding patterns in agarose gel electrophoresis that readi ...[more]