Ontology highlight
ABSTRACT:
SUBMITTER: Kim S
PROVIDER: S-EPMC5290157 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
Kim Soohong S De Jonghe Joachim J Kulesa Anthony B AB Feldman David D Vatanen Tommi T Bhattacharyya Roby P RP Berdy Brittany B Gomez James J Nolan Jill J Epstein Slava S Blainey Paul C PC
Nature communications 20170127
Low-cost shotgun DNA sequencing is transforming the microbial sciences. Sequencing instruments are so effective that sample preparation is now the key limiting factor. Here, we introduce a microfluidic sample preparation platform that integrates the key steps in cells to sequence library sample preparation for up to 96 samples and reduces DNA input requirements 100-fold while maintaining or improving data quality. The general-purpose microarchitecture we demonstrate supports workflows with arbit ...[more]