Ontology highlight
ABSTRACT:
SUBMITTER: Liu X
PROVIDER: S-EPMC5290280 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
Nature communications 20170130
Protein ubiquitination is mediated sequentially by ubiquitin activating enzyme E1, ubiquitin conjugating enzyme E2 and ubiquitin ligase E3. Uba1 was thought to be the only E1 until the recent identification of Uba6. To differentiate the biological functions of Uba1 and Uba6, we applied an orthogonal ubiquitin transfer (OUT) technology to profile their ubiquitination targets in mammalian cells. By expressing pairs of an engineered ubiquitin and engineered Uba1 or Uba6 that were generated for excl ...[more]