Ontology highlight
ABSTRACT:
SUBMITTER: Reatini BS
PROVIDER: S-EPMC5290727 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
Reatini Bryan S BS Ensink Elliot E Liau Brian B Sinha Jessica Y JY Powers Thomas W TW Partyka Katie K Bern Marshall M Brand Randall E RE Rudd Pauline M PM Kletter Doron D Drake Richard R Haab Brian B BB
Analytical chemistry 20161115 23
Glycans are critical to protein biology and are useful as disease biomarkers. Many studies of glycans rely on clinical specimens, but the low amount of sample available for some specimens limits the experimental options. Here we present a method to obtain information about protein glycosylation using a minimal amount of protein. We treat proteins that were captured or directly spotted in small microarrays (2.2 mm × 2.2 mm) with exoglycosidases to successively expose underlying features, and then ...[more]