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Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein.

ABSTRACT: A variety of organisms have evolved mechanisms to detect and respond to light, in which the response is mediated by protein structural changes after photon absorption. The initial step is often the photoisomerization of a conjugated chromophore. Isomerization occurs on ultrafast time scales and is substantially influenced by the chromophore environment. Here we identify structural changes associated with the earliest steps in the trans-to-cis isomerization of the chromophore in photoactive yellow protein. Femtosecond hard x-ray pulses emitted by the Linac Coherent Light Source were used to conduct time-resolved serial femtosecond crystallography on photoactive yellow protein microcrystals over a time range from 100 femtoseconds to 3 picoseconds to determine the structural dynamics of the photoisomerization reaction.

SUBMITTER: Pande K 

PROVIDER: S-EPMC5291079 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature

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Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein.

Pande Kanupriya K   Hutchison Christopher D M CD   Groenhof Gerrit G   Aquila Andy A   Robinson Josef S JS   Tenboer Jason J   Basu Shibom S   Boutet Sébastien S   DePonte Daniel P DP   Liang Mengning M   White Thomas A TA   Zatsepin Nadia A NA   Yefanov Oleksandr O   Morozov Dmitry D   Oberthuer Dominik D   Gati Cornelius C   Subramanian Ganesh G   James Daniel D   Zhao Yun Y   Koralek Jake J   Brayshaw Jennifer J   Kupitz Christopher C   Conrad Chelsie C   Roy-Chowdhury Shatabdi S   Coe Jesse D JD   Metz Markus M   Xavier Paulraj Lourdu PL   Grant Thomas D TD   Koglin Jason E JE   Ketawala Gihan G   Fromme Raimund R   Šrajer Vukica V   Henning Robert R   Spence John C H JC   Ourmazd Abbas A   Schwander Peter P   Weierstall Uwe U   Frank Matthias M   Fromme Petra P   Barty Anton A   Chapman Henry N HN   Moffat Keith K   van Thor Jasper J JJ   Schmidt Marius M  

Science (New York, N.Y.) 20160505 6286

A variety of organisms have evolved mechanisms to detect and respond to light, in which the response is mediated by protein structural changes after photon absorption. The initial step is often the photoisomerization of a conjugated chromophore. Isomerization occurs on ultrafast time scales and is substantially influenced by the chromophore environment. Here we identify structural changes associated with the earliest steps in the trans-to-cis isomerization of the chromophore in photoactive yello  ...[more]

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