Dataset Information

Repeated hapten exposure induces persistent tactile sensitivity in mice modeling localized provoked vulvodynia.

ABSTRACT:

Background

Vulvodynia is a remarkably prevalent chronic pain condition of unknown etiology. Epidemiologic studies associate the risk of vulvodynia with a history of atopic disease. We used an established model of hapten-driven contact hypersensitivity to investigate the underlying mechanisms of allergy-provoked prolonged sensitivity to pressure.

Methods

We sensitized female ND4 Swiss mice to the hapten oxazolone on their flanks, and subsequently challenged them four days later with oxazolone or vehicle for ten consecutive days on the labia. We evaluated labiar sensitivity to touch, local mast cell accumulation, and hyperinnervation after ten challenges.

Results

Oxazolone-challenged mice developed significant tactile sensitivity that persisted for over three weeks after labiar allergen exposures ceased. Allergic sites were characterized by mast cell accumulation, sensory hyper-innervation and infiltration of regulatory CD4+CD25+FoxP3+ T cells as well as localized early increases in transcripts encoding Nerve Growth Factor and nerve-mast cell synapse marker Cell Adhesion Molecule 1. Local depletion of mast cells by intra-labiar administration of secretagogue compound 48/80 led to a reduction in both nerve density and tactile sensitivity.

Conclusions

Mast cells regulate allergy-provoked persistent sensitivity to touch. Mast cell-targeted therapeutic strategies may provide novel means to manage and limit chronic pain conditions associated with atopic disease.

SUBMITTER: Landry J

PROVIDER: S-EPMC5291437 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Repeated hapten exposure induces persistent tactile sensitivity in mice modeling localized provoked vulvodynia.

Landry Jasmine J Martinov Tijana T Mengistu Hanna H Dhanwada Jyothi J Benck Charles J CJ Kline Jaclyn J Boo Beebie B Swanson Linnea L Tonc Elena E Daughters Randy R Fife Brian T BT Chatterjea Devavani D

PloS one 20170203 2

<h4>Background</h4>Vulvodynia is a remarkably prevalent chronic pain condition of unknown etiology. Epidemiologic studies associate the risk of vulvodynia with a history of atopic disease. We used an established model of hapten-driven contact hypersensitivity to investigate the underlying mechanisms of allergy-provoked prolonged sensitivity to pressure.<h4>Methods</h4>We sensitized female ND4 Swiss mice to the hapten oxazolone on their flanks, and subsequently challenged them four days later wit ...[more]

PMID: 28158195

Similar Datasets

Project description:BackgroundRecent studies have shown that neural stimulation can be used to provide artificial sensory feedback to amputees eliciting sensations referred on the amputated hand. The temporal properties of the neural stimulation modulate aspects of evoked sensations that can be exploited in a bidirectional hand prosthesis.MethodsWe previously collected evidence that the derivative of the amplitude of the stimulation (intra-digit temporal dynamics) allows subjects to recognize object compliance and that the time delay among stimuli injected through electrodes implanted in different nerves (inter-digit temporal distance) allows to recognize object shapes. Nevertheless, a detailed characterization of the subjects' sensitivity to variations of intra-digit temporal dynamic and inter-digit temporal distance of the intraneural tactile feedback has not been executed. An exhaustive understanding of the overall potentials and limits of intraneural stimulation to deliver sensory feedback is of paramount importance to bring this approach closer and closer to the natural situation. To this aim, here we asked two trans-radial amputees to identify stimuli with different temporal characteristics delivered to the same active site (intra-digit temporal Dynamic Recognition (DR)) or between two active sites (inter-digit Temporal distance Recognition (TR)). Finally, we compared the results achieved for (simulated) TR with conceptually similar experiments with real objects with one subject.ResultsWe found that the subjects were able to identify stimuli with temporal differences (perceptual thresholds) larger than 0.25?s for DR and larger than 0.125?s for TR, respectively. Moreover, we also found no statistically significant differences when the subjects were asked to identify three objects during simulated 'open-loop' TR experiments or real 'closed-loop' tests while controlling robotic hand.ConclusionsThis study is a new step towards a more detailed analysis of the overall potentials and limits of intraneural sensory feedback. A full characterization is necessary to develop more advanced prostheses capable of restoring all lost functions and of being perceived more as a natural limb by users.

Dataset Information

Repeated hapten exposure induces persistent tactile sensitivity in mice modeling localized provoked vulvodynia.

Background

Methods

Results

Conclusions

Publications

Repeated hapten exposure induces persistent tactile sensitivity in mice modeling localized provoked vulvodynia.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure