Ontology highlight
ABSTRACT:
SUBMITTER: Nyffenegger C
PROVIDER: S-EPMC5291517 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Nyffenegger Christian C Nordvang Rune Thorbjørn RT Jers Carsten C Meyer Anne S AS Mikkelsen Jørn Dalgaard JD
PloS one 20170203 2
A sialidase (EC 3.2.1.18) from the non-pathogenic Trypanosoma rangeli, TrSA, has been shown to exert trans-sialidase activity after mutation of five specific amino acids in the active site (M96V, A98P, S120Y, G249Y, Q284P) to form the so-called TrSA5mut enzyme. By computational and hypothesis driven approaches additional mutations enhancing the trans-sialidase activity have been suggested. In the present work, we made a systematic combination of these mutations leading to seven new variants of t ...[more]